魔芋组织培养中褐变成因的探讨  

Studies on factors of browning in the tissue culture of Amorphophallus rivieri Durieu

作　　者：王玲[1] 房亚南[1] 马继琼[1] 李勇军[1] 

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,云南昆明650223

出　　处：《西南农业学报》2006年第4期719-721,共3页Southwest China Journal of Agricultural Sciences

基　　金：云南省科技厅"十五"攻关项目(2001NG59)

摘　　要：研究了魔芋在组培时制约其成功的瓶颈问题——褐变的成因。结果表明，培养物的生理状态，培养的光、温条件及外源激素的种类、水平、组合影响了培养物酚类物质的多少、多酚氧化酶的活性，从而决定了培养物的褐变程度．通过试验比较，一龄芋的顶芽在培养基MS＋6BA 1mg／L＋NAA 1mg／L＋4．5％琼脂，温度25～30oC、光照1500lx的条件下培养物褐变率仅为6％，愈伤组织诱导率达94％，成功率最高。Factors of browning formation in the tissue culture of Amorphophallus rivieri Durieu were studied. The results showed that ： （i）Physiological state of explants, cultural conditions of temperature and light and types, content and combination of hormone affected actives of phenol Oxidases, content of phenolic compounds and degrees of browning in explants. （ii） Browning rates of apical buds of one year old Amorphophallus rivieri Durieu were only 6 % and inductive rates of callus were 94 % higher in MS ＋ 1.0 mg·L^-1 6BA ＋ 1.0 1.0 mg·L^-1 , NAA ＋ 4.5 % agar and contritions of light 1500 lx and temperature 25 - 30 ℃.

关 键 词：魔芋 组织培养 褐变 酚类物质 多酚氧化酶 

分 类 号：S632[农业科学—蔬菜学]