热带假丝酵母木糖醇脱氢酶的分离纯化  

Purification of Xylitol Dehydrogenase from Candida tropicalis

作　　者：何圣楠[1] 林影[1] 吴晓英[1] 陈璐菲[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广州510641

出　　处：《食品与发酵工业》2006年第7期46-49,共4页Food and Fermentation Industries

基　　金：广东省自然科学基金资助项目(04020051)

摘　　要：探讨了热带假丝酵母中木糖醇脱氢酶的分离纯化工艺。分步采用硫酸铵分级沉淀、DEAE-Sepharose离子交换层析、Sephacryl S-200凝胶层析以及Hi Trap Blue HP亲和层析等纯化方法,得到电泳纯的木糖醇脱氢酶。经SDS-PAGE和native-PAGE分析表明,木糖醇脱氢酶分子质量约为90ku,由2个分子质量为45ku的亚基组成。最终酶蛋白纯化倍数达到60·8倍,回收率为10·9%。Xylitol dehydrogenase （XDH） is one of the key enzymes in the conversion of xylose to ethanol. XDH from Candida tropicalis was purified by ammonium sulphate precipitation, DEAE Sepharose F.F. ion exchange chromatography, Sephacryl S-200 gel filtration and HiTrap Blue HP affinity chromatography. Purified XDH following the above four steps was 60.8-fold and the activity recovery was 10.9% . The purified enzyme was composed of two identical subunits with.

关 键 词：热带假丝酵母 木糖醇脱氢酶 纯化 

分 类 号：TS201.3[轻工技术与工程—食品科学]