辛伐他汀对肿瘤坏死因子α诱导的人脐静脉内皮细胞基质金属蛋白酶9表达的影响  

Effect of Simvastatin on Expression of Matrix Metalloproteinase 9 Induced by Tumor Necrosis Factor α in Human Umbilical Vein Endothelial Cell

作　　者：张承俊[1] 朱兴雷[2] 

机构地区：[1]济南市第一人民医院,山东省济南市250011 [2]山东省立医院,山东省济南市250012

出　　处：《中国动脉硬化杂志》2006年第1期50-52,共3页Chinese Journal of Arteriosclerosis

摘　　要：目的观察辛伐他汀对肿瘤坏死因子α诱导的人脐静脉内皮细胞基质金属蛋白酶9表达的影响,以期探讨辛伐他汀可能的非调脂抗动脉粥样硬化的作用。方法选用体外培养的人脐静脉内皮细胞加20μg/L肿瘤坏死因子α及0.01、0.1、1.0和10μmol/L辛伐他汀共同孵育6 h1、2 h和24 h,采用免疫组织化学和逆转录聚合酶链反应分别测定基质金属蛋白酶9的免疫荧光及mRNA表达水平。结果肿瘤坏死因子α可明显诱导人脐静脉内皮细胞基质金属蛋白酶9的表达,辛伐他汀对肿瘤坏死因子α诱导的基质金属蛋白酶9 mRNA的表达有明显的抑制作用,且呈浓度依赖性和时间依赖性。结论辛伐他汀抑制肿瘤坏死因子α诱导的人脐静脉内皮细胞基质金属蛋白酶9的表达,可能对抗动脉粥样硬化、稳定硬化斑块、防治斑块破裂和预防急性冠状动脉综合征的发生产生有益的作用。Aim To observe the influence of simvastafin on tumor necrosis factor α（ TNF-α）-induced expression of metalloproteinase-9（MMP-9）in human umbilical vein endothelial cell（hUVEC）and to investigate the non-lipid mechanisms of 3- hydroxy-3-methyl-glutaryl coenzyme A（HMG-CoA） reductase inhibitors on anti-atheroselerosis. Methods The expression of MMP-9 in protein level and mRNA level was detected with immunohistochemistry and reverse transcription polymerase chain reaction（ RT-PCR）. Results TNF-α could induce the expression of MMP-9 in hUVEC. Simvastatin could inhibit the expression of MMP-9 and their inhibitory effects were concentration-dependent and time-dependent. Conclusion The inhibition of TNF-α-induced MMP-9 expression in hUVEC by simvastatin may crucially contribute to the clinical benefits of HMG-CoA reductase inhibitors on coronary artery disease, independent of cholesterol-lowing effects.

关 键 词：病理学与病理生理学 他汀类药物的抗动脉粥样硬化作用 逆转录聚合酶链反应 辛伐他汀 人脐静脉内皮细胞 肿瘤坏死因子Α 基质金属蛋白酶9 

分 类 号：R363[医药卫生—病理学]