半边莲生物碱抑制内皮素诱导的大鼠主动脉平滑肌细胞增殖  

Lobelia Chinensis Lour Alkaloids Inhibiting Rat Aortic Vascular Smooth Muscle Cell Proliferation Induced by Endothelin

作　　者：王婧婧[1] 范秀珍[2] 刘尚明[1] 魏欣冰[1] 任冬梅[3] 陈融[1] 李莉[1] 胡维诚[1] 

机构地区：[1]山东大学医学院,山东省济南市250012 [2]山东大学护理学院,山东省济南市250012 [3]山东大学药学院,山东省济南市250012

出　　处：《中国动脉硬化杂志》2006年第2期107-110,共4页Chinese Journal of Arteriosclerosis

基　　金：教育部高等学校博士学科点专项科研基金资助项目(No.39730210);山东省自然科学基金资助项目(No.Q2004C03)

摘　　要：目的研究半边莲生物碱对内皮素诱导的血管平滑肌细胞增殖的作用。方法用内皮素1刺激大鼠主动脉平滑肌细胞增殖，以细胞计数试剂盒计数细胞个数、氚标胸腺嘧啶脱氧核苷掺入检测DNA的合成量、免疫细胞化学技术观察增殖细胞核抗原的表达活性作为细胞增殖的指标，并应用台盼兰拒染、乳酸脱氢酶检测观察半边莲生物碱的细胞毒性反应。结果内皮素1（10^-7 mol／L）可明显促进细胞增殖（与对照组相比，P〈0.01）；半边莲生物碱和BQ-123均可抑制内皮素1所诱导的细胞增殖（与内皮素1组相比，P〈0，05）；半边莲生物碱的抑制作用与浓度存在明显的依赖关系，但对活细胞数目和乳酸脱氢酶释放量均没有影响（P〉0，05）。结论半边莲生物碱（50～200mg／L）可浓度依赖性地抑制内皮素1所诱导的大鼠主动脉平滑肌细胞增殖，且此抑制作用并非是通过细胞毒性作用实现的。Aim To determine the effect of scutel laria barbata alkaloid （LCLAs）on proliferation of cultured vascular smooth muscle cell （VSMC）induced by endothelin（ET）. Methods Rat aortic VSMC was cultured and divided into four groups： ET group, ET＋ LCLAs group, ET ＋ BQ-123 （the selective ETA receptor antagonist） group and control group, then the cell proliferation activity was subsequently quantified by cell counting kit-8 to count cell number, ^3H -TdR incorporation to measure DNA synthesis and quantitative immunohistochemical technique to investigate the expression of proliferating cell nuclear antigen （PCNA）in VSMC. Cytotoxicity was measured by Lactate Dehydrogenase （LDH）colofimetry and Trypan blue exclusion tests. Results Compared with the control group, ET- 1 （ 10.7 mol/ L）significantly enhanced the proliferation activity of VSMC（ P 〈 0. 01 ）, 50 mg/L LCLAs inhibited the increase of cell number（ 6.01 ± 0.05, P 〈 0.05 ） and ^3H-TdR incorporation（ 1 464 ± 45 counts/rain, P 〈 0.05）compared with ET group, but has no evident effect on the proliferation activity of PCNA. 100, 200 mg/L LCI.As and 10^6 mol/L BQ-123 markedly decrease the cell number（5.73 ±0.09, 4.81 ± 0.08, 4.77 ± 0.06, respectively）, ^3H TdR incorporation（ 1 263±1, 1 129±49, 1 140± 56 counts/rain, respectively）and the expression of PCNA（350 ± 29, 341 ± 53, 336 ± 39, respectively）compared with ET group（P〈0.01）. Conclusions LCLAs（50-200mg/L）inhibited ET-induced proliferation of VSMC in a dose-dependent manner and the effect was not due to nonspecific cytotoxicity.

关 键 词：病理学与病理生理学 半边莲生物碱对血管平滑肌细胞增殖的影响 免疫细胞化学 半边莲生物碱 内皮素 增殖细胞核抗原 增殖 

分 类 号：R363[医药卫生—病理学]