提高红曲菌9903A原生质体形成数量和再生率的研究  

STUDY ON FORMATION AND REGENERATION OF PROTOPLASTS FROM MONASCUS PURPUREUS 9903A

作　　者：张垸帼[1] 段开红[2] 牛丹丹[1] 许赣荣[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214036 [2]内蒙古农业大学生物工程学院,内蒙古呼和浩特010018

出　　处：《食品研究与开发》2006年第8期54-58,共5页Food Research and Development

摘　　要：采用酶解法制备红曲菌9903A原生质体。研究红曲菌菌丝体的菌龄、酶种类、酶解温度、酶解时间、稳渗剂和几种再生培养基对原生质体形成和再生的影响。确定酶解最佳条件为:菌龄50h～60h,组合酶(蜗牛酶(0.6%)+溶菌酶(0.4%)+纤维素酶(0.8%)),酶解温度31℃,酶解时间1.5h。研究了稳渗剂对原生质体制备与再生的影响,并对红曲菌9903A原生质体的释放和再生过程进行了观察。结果表明:山梨醇可促进原生质体的释放;蔗糖对原生质体的再生有促进作用。在此条件下原生质体的形成为4.24×108/mL,再生率为52%。In the study, the protoplasts of Monascus purpureus 9903A was prepared by enzyme digesting method. The effects of some factors such as the mycelium age of Monascus, osmotic stabilizers, different combinations of enzyme mixture and digesting temperature and time, on protoplast formation from the Monascus purpureus 9903A were investigated. The results showed that the protoplasts with higher yield and quality were obtained by treating the spores with 31℃ and mixture of 1.8 % enzymes （Snailase （0.6 %） ＋Lysozyme （0.4 %）＋Cellulase（0.8 %）） for 1.5 h. The spores from 50 h～60 h were appropriate for preparation of protoplasts; Sorbitol can promote the formation of protoplast and sucrose can facilitate the regeneration of protoplast. As a result, quantity of formation of protoplasts was 4.24 × 10^8/mL. The regeneration ratio is 52 %. This laid the foundation of efficient protoplasts regeneration from Monascus and cell fusion.

关 键 词：红曲霉 原生质体 形成 再生 

分 类 号：Q933[生物学—微生物学]