单扫描极谱法测定保健食品中绿原酸  

Determination of chlorogenic acid in healhth foods by single-sweeping polarographic analysis

作　　者：彭科怀[1] 赵年华[1] 汤晓勤[1] 郑娟娟[2] 

机构地区：[1]四川省疾病预防控制中心,成都610045 [2]华西公共卫生学院,成都610041

出　　处：《中国卫生检验杂志》2006年第9期1048-1049,1061,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：研制保健食品中绿原酸的单扫描极谱分析方法。方法：样品经50％的乙醇溶液超声振荡提取，用拟定的极谱方法进行定性和定量测定。结果：在0．05mol／L硫酸、3mol／L亚硝酸钠、2．5mol／L乙酸钠介质中，绿原酸峰电位为-172mV（vs．SCE）。绿原酸浓度在5．0-50．0μg之间线性关系良好，相关系数r=0．9993，回归方程Y：2．6923X-2.365。相对标准偏差（RSD）为1．70％～6．66％（n=5）。加标回收率为94．3％～97．1％（n=9）。结论：建立的单扫描极谱法测定绿原酸的方法简便快速、结果准确、灵敏度高、仪器廉价、易于普及。适于实验室检测样品中绿原酸的含量。Objective： Single - sweeping polarograph for the analysis method of chlorogenic acid in health foods was studied. Methods ：The chlorogenic acid was determined by the method qualitatively and quantitatively. Results：In the supporting electrolyte solution that composed of 0. 05 mol/L sulfuric acids, 3 mol/L sodium nitrite, 2.5 mol/L sodium acetate, the peak potential of chlorogenic acid was - 172 mV（ vs. SCE）. The peak current of chlorogenic acid was linear to the concentration of chlorogenic acid in the range of 5.0 - 50. 0 μg. The coefficient of correlation was r = 0. 9993. The regression equation was Y = 2. 6923X -2. 365. Its relative standard deviation （RSD） was in the range of 1.70% -6. 66% （n = 5）. Recovery of chlorogenic acid was in the range of 94. 3% - 97. 1% （ n = 9）. Conclusion ： The method of single - sweeping polarograph for the determination of chlorogenic acid is simple, rapid and the results are accurate and highly sensitive. The single - sweeping polarograph is cheap and easy to popularize. The method can be applied to the determination of chlorogenic acid in lab.

关 键 词：极谱法 绿原酸 保健食品 

分 类 号：O657.14[理学—分析化学]