Protective Effects of Focal Ischemic Preconditioning and HSP70 Expression on Middle Cerebral Artery Occlusion in Rats  

作　　者：赵建华 孙圣刚 陈小武 

机构地区：[1]Department of Neurology,Union Hospital,Tongji Medical College,Huagzhong University of Science and Technology,Wuhan 430022,China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2006年第4期436-439,共4页华中科技大学学报（医学英德文版）

摘　　要：Summary： To systematically evaluate the importance of protein synthesis in ischemic preconditioning （PC）-induced ischemic tolerance （IT）, temporary middle cerebral artery occlusion （MCAO） by Longa （20 min） was used for PC （ischemic precondioning）. Twenty-four hours of reperfusion was allowed after PC and before permanent MCAO to establish ischemic tolerance （IT） to compare with non-PC （sham-operated） rats （n=5 for each group）. Infarct size and neurological deficits were measured 24 h after PMCAO. Samples of brain were taken for the determination of HSP70 expression by Western blot analysis. The effects of the protein synthesis inhibitor cycloheximide administered just before PC or administered long after PC but just before PMCAO on IT were also determined （n=5 for each group）. Our results showed that hemispheric infarct was significantly reduced （P〈0.01） only if PC was performed after 24 h, and PC significantly （P〈0.05） reduced neurological deficits （similar to reductions in infarct size）. Cycloheximide eliminated ischemic PC-induced IT effects on both brain injury and neurological deficits if administered before PC but not if administered long after PC but before PMCAO. PC produced no brain injury but did increase HSP70 protein 24 h after PC. Cycloheximide eliminated that effect. The results suggest that PC is a powerful inducer of ischemic brain tolerance as reflected by the preservation of brain tissue and motor function. PC induces IT that is dependent on de novo protein synthesis.Summary： To systematically evaluate the importance of protein synthesis in ischemic preconditioning （PC）-induced ischemic tolerance （IT）, temporary middle cerebral artery occlusion （MCAO） by Longa （20 min） was used for PC （ischemic precondioning）. Twenty-four hours of reperfusion was allowed after PC and before permanent MCAO to establish ischemic tolerance （IT） to compare with non-PC （sham-operated） rats （n=5 for each group）. Infarct size and neurological deficits were measured 24 h after PMCAO. Samples of brain were taken for the determination of HSP70 expression by Western blot analysis. The effects of the protein synthesis inhibitor cycloheximide administered just before PC or administered long after PC but just before PMCAO on IT were also determined （n=5 for each group）. Our results showed that hemispheric infarct was significantly reduced （P〈0.01） only if PC was performed after 24 h, and PC significantly （P〈0.05） reduced neurological deficits （similar to reductions in infarct size）. Cycloheximide eliminated ischemic PC-induced IT effects on both brain injury and neurological deficits if administered before PC but not if administered long after PC but before PMCAO. PC produced no brain injury but did increase HSP70 protein 24 h after PC. Cycloheximide eliminated that effect. The results suggest that PC is a powerful inducer of ischemic brain tolerance as reflected by the preservation of brain tissue and motor function. PC induces IT that is dependent on de novo protein synthesis.

关 键 词：ischemic precondioning ischemic tolerance brain infarct HSP70 CYCLOHEXIMIDE 

分 类 号：R743[医药卫生—神经病学与精神病学]