青蒿素与氯化血红素作用的荧光研究及分析应用  

Fluorescence Investigation on Interaction between Artemisinin (Qinghaosu) and Hemin and Its Analytical Application

作　　者：陈莉华[1] 尹红[1] 杨朝霞[1] 张克梅[1] 刘六战[2] 沈含熙[2] 

机构地区：[1]吉首大学化学化工学院,湖南吉首416000 [2]南开大学化学院,天津300071

出　　处：《光谱学与光谱分析》2006年第9期1640-1643,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(20075012);湖南省教委科研基金(02C313)资助

摘　　要：介绍了吡罗红为指示剂通过荧光降低法研究青蒿素与氯化血红素之间的相互作用。实验发现两者为酶和底物作用模型，作用位点为药物的过氧基团和氯化血红素活性中心的金属离子，其动力学催化常数Km，Vmax和Kcat分别为8．4×10^-5mol·L^-1，7．4×10^-6mol·L^-1·s^-1及50．23s^-1，催化活性分别受去激活剂和高温影响。在最佳条件下，荧光降低值△F（F0-F）与青蒿素浓度在0．0～1．27×10^-6mol·L^-1范围内成线性关系，检测限为2．3×10^-8mol·L^-1，该方法已用于测定血浆和尿液介质中的微量青蒿素。Heroin-catalytic decomposition of artemisinin （qinghaosu, QHS） was studied using pyronine B （PB） as an indicator. The interaction between heroin and QHS was an enzyme-substrate model, and the action sites were the endoperoxide moiety of QHS and the central metal ion of enzyme respectively. The kinetic catalytic constant depends upon enzyme and substrate concentrations, and the Michaelis-Menten parameters Km, Vmax and Kcat was 8. 4 × 10^-5 mol·L^-1 , 7.4 × 10^-6 mol·L^-1 s^-1 and 50.23 s^-1 respectively. The catalytic activity of hemin was inhibited in the presence of deactivated agents and at high temperature. Under optimal conditions, the change in fluorescence intensity （F0-F） of pyronine B was proportional to the QHS concentration from 0. 0 to 1.27×10^-6 mol·L^-1 , and the detection limit （3σ） was as low as 2.3^10^-8 mol·L^-1. The proposed method was applied to detect the concentration of QHS in the media of plasma and urine.

关 键 词：青蒿素 氯化血红素 抗疟活性 荧光降低法 

分 类 号：O657.3[理学—分析化学]