生物表面活性剂产生菌犁头霉菌(Absidia orchidis)的筛选及发酵条件优化  

Screening and optimization of cultural medium of bio-surfactant strain Absidia orchidis

作　　者：卢国满[1] 刘红玉[1] 曾光明[1] 钟华[1] 时进钢[1] 张林达[1] 

机构地区：[1]湖南大学环境科学与工程学院,长沙410082

出　　处：《环境科学学报》2006年第9期1426-1432,共7页Acta Scientiae Circumstantiae

基　　金：国家高技术研究发展计划(863)项目(No.2004AA649370);湖南省自然科学基金(No.05JJ30021)~~

摘　　要：采用植物油为唯一碳源,设计选择培养基,从饭店下水道污泥中筛选出生物表面活性剂产生菌.结果分离到12株菌,其中一株能使发酵液的表面张力值从68mN·m-1降低到34.5mN·m-1,具有开发潜力,被选出作进一步的研究.该菌株经鉴定为犁头霉菌(Absidiaorchidis).通过正交试验对犁头霉菌的培养条件进行优化,其优化培养条件为植物油3.6g·L-1,KH2PO412.1g·L-1,Na2HPO45g·L-1,(NH4)2SO41g·L-1,NaNO32g·L-1,酵母浸膏0.1g·L-1,MgSO·47H2O0.15g·L-1,NaCl5g·L-1,CaCl20.1g·L-1,EDTA1g·L-1,KI0.83μg·L-1,H3PO40.01μg·L-1,CoCl·26H2O0.048μg·L-1,MnSO4·H2O0.312μg·L-1,Na2MoO·42H2O0.048μg·L-1,ZnCl20.287μg·L-1,CuSO·45H2O0.125μg·L-1,初始pH值8,接种量6%.发酵70h时可获得生物表面活性剂的最大收获量,此时发酵液中生物表面活性剂的相对浓度达402倍.Bio-surfactant strains were screened from the sludge of a restaurant by designing a selective cultural medium with vegetable oil as its sole carbon source. 12 strains which could produce bio-surfactant were obtained. One of them which could reduce the surface tension of the fermentation solution from 68 to 34.5 mN m ^- 1 was chosen for further study because of its application potential. This strain was identified as Absidia orchidis. The cultural conditions of Absidia orchidis were optimized by using orthogonal experimental method. The optimized fermentation conditions were： vegetable oil 3.6 g·L ^- 1 , KH2PO4 12.1 g·L^-1, Na2 HPO45 g·L^-1, （NH4）2SO41 g·L^-1, NaNO32 g·L^-1, yeast paste 0. 1 g·L^-1, MgSO4·7H2O 0. 15 g·L^-1, NaCI 5 g·L^-1, CaCl2 0.1 g·L^-1, EDTA 1 g·L^-1, K10.83 μg·L^-1, H3PO4 0.01 μg·L^-1, COCl2.6H2O0.048 μg·L^-1, MnSO4·H2O 0.312 μg·L^-1 Na2 MoO42·H2O0. 048μg·L^-1 ZnCI2 0. 287 μg·L^-1 CuSO4.5H2O 0. 125 μg· L^-1 with initial pH 8, and inoculating dose 6%. The highest bio- surfactant production could be obtained by fermenting for 70 hours under this condition with Relative Biosurfactant Concentration to be 402.

关 键 词：生物表面活性剂 菌株筛选 正交实验 培养条件优化 犁头霉菌 

分 类 号：X172[环境科学与工程—环境科学]