SENSITIVITY OF LEUKEMIC CELL LINE HL-60 TO COMBINATION OF NEFERINE AND ARSENIC  

作　　者：刘革修 张洹 何冬梅 

机构地区：[1]Institute of Hematology, Jinan University Medical College, Guangzhou 510632

出　　处：《Chinese Journal of Cancer Research》2006年第3期183-187,共5页中国癌症研究（英文版）

基　　金：This work was supported by the Grant from Natural Science Foundation of Guangdong Province (No. 04010446).

摘　　要：Objective： To determine whether neferine （Nef） enhances the sensitivity of human myeloid leukemia HL-60 cells to arsenic trioxide （ATO）. Methods： Apoptosis was detected by DNA electrophoresis. Giemsa staining was used to observe the apoptotic cells under microscope. The apoptotic rates of cells were analyzed using flow cytometry. The inhibitory rates of cell growth were assayed by MTT, and the expression of P-gp was determined by flow cytometry. Results： Low doses of ATO （1.0 μmol/L） only partially inhibitory percentage of cell growth at 72 h was （9.92±3.03） % （P〉0.05, vs control）. The combination of 1.0 μmol/L ATO with 2.0 μmol/L Nef inhibited （45.27±4.93） % of cell growth, and induced apoptosis in leukemic cells more significantly than wither ATO or Nef on their own （P〈0.01）. Moreover, ATO-induced expression of P-gp in leukemic cells was inhibited significantly by Nef. Conclusion： These results indicate that Nef significantly increases sensitivity of leukemic cells to ATO, which might be associated with inhibitory expression of P-gp gene. Combined use of the two agents could be a novel and attractive strategy in leukemia treatment.Objective： To determine whether neferine （Nef） enhances the sensitivity of human myeloid leukemia HL-60 cells to arsenic trioxide （ATO）. Methods： Apoptosis was detected by DNA electrophoresis. Giemsa staining was used to observe the apoptotic cells under microscope. The apoptotic rates of cells were analyzed using flow cytometry. The inhibitory rates of cell growth were assayed by MTT, and the expression of P-gp was determined by flow cytometry. Results： Low doses of ATO （1.0 μmol/L） only partially inhibitory percentage of cell growth at 72 h was （9.92±3.03） % （P〉0.05, vs control）. The combination of 1.0 μmol/L ATO with 2.0 μmol/L Nef inhibited （45.27±4.93） % of cell growth, and induced apoptosis in leukemic cells more significantly than wither ATO or Nef on their own （P〈0.01）. Moreover, ATO-induced expression of P-gp in leukemic cells was inhibited significantly by Nef. Conclusion： These results indicate that Nef significantly increases sensitivity of leukemic cells to ATO, which might be associated with inhibitory expression of P-gp gene. Combined use of the two agents could be a novel and attractive strategy in leukemia treatment.

关 键 词：NEFERINE APOPTOSIS Arsenic trioxide LEUKEMIA 

分 类 号：R733.7[医药卫生—肿瘤]