酸化法提取海带多糖及其纯化的研究  

Studies on extraction and purification of laminarin by acid solubilization

作　　者：周裔彬[1] 汪东风[1] 杜先锋[2] 倪晓波[2] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]安徽农业大学食品科学与工程系,安徽合肥230036

出　　处：《南京农业大学学报》2006年第3期103-107,共5页Journal of Nanjing Agricultural University

基　　金：安徽省教育厅自然科学基金项目(2005KJ177)

摘　　要：建立了一套用酸化法提取、纯化海带中多糖类化合物的方法。结果表明:在75℃、4 h的反应条件下,海带多糖的提取率较高,粗糖可达35.10%;纯化后的多糖含量可达96.60%;经薄层层析、高效液相色谱、Sephadex G洗脱分析,海带多糖含有2-3种多糖,是一种混合多糖。A high efficient procedure was developed to extract laminarin by acid solubilization, and purify compounds from kelp in this paper. The results showed that the yield of laminarin was higher at 75℃ for 4 h. The total rate of crude laminarin was up to 35.10%. The content of laminarin was 96. 60% in the purified product, which contented 2 -3 polysaccharide components when analysed by thin layer chromatography, high performance liquid chromatography, Sephadex G.

关 键 词：海带多糖 提取 纯化 

分 类 号：S968.421[农业科学—水产养殖]