CXC CHEMOKINE RECEPTOR 3 MODULATES BLEOMYCIN-INDUCED PULMONARY INJURY VIA INVOLVING INFLAMMATORY PROCESS  

作　　者：Jin-ming Gao Bao Lu Zi-jian Guo 

机构地区：[1]Department of Respiratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 100730 [2]Ina Sue Perlmutter Laboratory, Division of Pulmonary, Children＇s Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115

出　　处：《Chinese Medical Sciences Journal》2006年第3期152-156,共5页中国医学科学杂志（英文版）

基　　金：Supported by the National Natural Sciences Foundation of China(30470767,30470768)

摘　　要：Objeelive To investigate the role of CXC chemokine receptor 3 （ CXCR3 ） in bleomycin-induced lung injury by using CXCR3 gene deficient mice. Methods Sex-, age-, and weight-matched C57BL/6 CXCR3 gene knockout mice and C57BL/6 wide type mice were challenged by injection of bleomycin via trachea. Lung tissue was stained with HE method. Airway resistance was measured. Bronchoalveolar lavage （BAL） was performed using phosphate buffered saline twice, cell number and differentials were counted by Diff-Quick staining. Interleukin （IL）-4, IL-5, IL-12p40, and interfon-~ in BAL fluid and lung homogenate were measured by enzyme-linked immunosorbent assay. Unpaired t test was explored to compare the difference between two groups. Results On day 7 after bleomycin injection via trachea, CXCR3 knockout mice were protected from bleomycininduced lung injury as evidenced by fewer accumulation of inflammatory cells in the airway and lung interstitium compared with their wild type littermates （ P 〈 0. 05 ）. Airway resistance was also lower in CXCR3 knockout mice compared with wild type mice （P 〈 0. 01 ）. Significantly lower level of inflammatory cytokines release, including the altered production of IL-4 and IL-5 both in BAL fluid and lung tissue was seen in CXCR3 knockout mice than in wild type mice （both P 〈0. 05）. Conclusion CXCR3 signaling promotes inflammatory cells recruiting and initiates inflammatory cytokines cascade following endotracheal bleomycin administration, indicating that CXCR3 might be a therapeutic target for pulmonary injury.

关 键 词：chemokine receptor BLEOMYCIN lung injury INFLAMMATION 

分 类 号：R563[医药卫生—呼吸系统]