间苯二酚法测定蜂蜜中果糖的研究  

Study on Determination Fructose in Honey by Resorcinol Method

作　　者：卢珍华[1] 倪辉[1] 杨远帆[1] 蔡慧农[1] 

机构地区：[1]集美大学生物工程学院,福建厦门361021

出　　处：《集美大学学报（自然科学版）》2006年第3期223-226,共4页Journal of Jimei University：Natural Science

基　　金：福建省自然科学基金资助项目(B0210026)

摘　　要：对利用间苯二酚法测定蜂蜜中果糖的条件进行了优化选择，并对其应用性进行了评估．结果表明，果糖与间苯二酚、盐酸共热生成的红色络合物在483nm处吸收峰最高；当间苯二酚质量浓度为2g／L、盐酸浓度为5mol／L、加热温度为100℃、加热时间为7min、果糖的质量浓度在0．2～2g／L之间时，果糖的质量浓度与吸光值具有良好的线性关系，回归方程为Y=0．035＋0．5648x，r^2=0．9994．In order to apply resorcinol spectrometric method to analyze fructose in honey, working conditions of the method were optimized and the validity was evaluated. The maxim absorption peak of the red complex resulting from the reaction of fructose, resorcinol and chloral acid occurred at 483 nm. When concentration of resorcinol was 2 g/L, concentration of chloral acid was 5 mol/L, temperature for reaction was 100℃, reaction time was 7 minutes, calibration curve displayed good linearity over the range of 0. 2 - 2 g/L with R^2 value of 0. 999 4, the regressive formula was Y = 0. 035 ＋ 0. 564 8x.

关 键 词：蜂蜜 果糖 间苯二酚 测定 

分 类 号：S896.1[农业科学—特种经济动物饲养]