双底物抑制下脂肪酶催化合成乳酸乙酯的研究  

Enzymatic esterification for ethyl lactate synthesis in acid and alcohol inhibition

作　　者：赵天涛[1] 张丽杰[1] 高静[2] 全学军[1] 

机构地区：[1]重庆工学院生物工程学院,重庆400050 [2]河北工业大学化工学院,天津300131

出　　处：《生物加工过程》2006年第3期51-55,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(20036010)

摘　　要：从微观体系考察了在双底物抑制下脂肪酶催化合成乳酸乙酯,叔丁醇是最佳溶剂,它可以很好地破坏乳酸分子由缔合而形成的分子簇。在所选的脂肪酶中,Novozym 435(南极假丝酵母脂肪酶B)的催化活性最好。综合产率和酶活两方面因素确定了最佳实验条件:酸醇摩尔比=1∶8、反应温度60℃、酸浓度0.3 mol/L、酶浓度45 g/mol、摇床转速200 r/min。Ethyl lactate was synthesized by lactic acid and ethanol in double substrates inhibition, and the best solvent was ten-butyl alcohol, which can break the force associated between lactic acid moleculars the view of microcosmic, and the best lipase is Novozym 435 （Lipase B from Candida antarctica ）. The optimized conditions were studied and confirmed in detail according to yield and activity of enzyme： the molar ratio （ acid： alcohol） was 1：8,the temperature was 60 ℃ ,the concentration of acid and lipase was 0.3 mol/L and 45 g/mol, respectively, the rotatation speed was 200 r/min.

关 键 词：双底物抑制 缔合 催化 脂肪酶 乳酸乙酯 

分 类 号：Q814[生物学—生物工程]