Characterization of Nocardia Plasmid pXT107  

作　　者：Hai-Yang XIA Yong-Qiang TIAN Ran ZHANG Kai-Chun LIN Zhong-Jun QIN 

机构地区：[1]College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China [2]Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China

出　　处：《Acta Biochimica et Biophysica Sinica》2006年第9期620-624,共5页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(No.30170019,30270030 and 30325003);the Natural Science Foundation of Shanghai(No.0202ZA14096);the Hi-tech Research and Development Program of China(No.2005AA227020)

摘　　要：Nocardia, Rhodococcus and Streptornyces, all members of the actinomycetes family, are Gram-positive eubacteria with high G＋C content and able to form mycelium. We report here a newly identified plasmid pXT107 of Nocardia sp. 107, one of the smallest circular plasmids found in Nocardia. The complete nucleotide sequence of pXT107 consisted of 4335 bp with 65% G＋C content, and encoded one replication extragenic palindromic （Rep） and six hypothetical proteins. The Rep, double-strand origin and single-strand origin of pXT107 resembled those of typical rolling-circle-replication plasmids, such as pNI 100 of Nocardia, pRE8424 of Rhodococcus and plJ 101 of Streptomyces. The Escherichia coli-Nocardia shuttle plasmid pHAQ22, containing the rep gene of pXT107, is able to propagate in Nocardia but not in Streptomyces.Nocardia, Rhodococcus and Streptornyces, all members of the actinomycetes family, are Gram-positive eubacteria with high G＋C content and able to form mycelium. We report here a newly identified plasmid pXT107 of Nocardia sp. 107, one of the smallest circular plasmids found in Nocardia. The complete nucleotide sequence of pXT107 consisted of 4335 bp with 65% G＋C content, and encoded one replication extragenic palindromic （Rep） and six hypothetical proteins. The Rep, double-strand origin and single-strand origin of pXT107 resembled those of typical rolling-circle-replication plasmids, such as pNI 100 of Nocardia, pRE8424 of Rhodococcus and plJ 101 of Streptomyces. The Escherichia coli-Nocardia shuttle plasmid pHAQ22, containing the rep gene of pXT107, is able to propagate in Nocardia but not in Streptomyces.

关 键 词：NOCARDIA PLASMID complete nucleotide sequence REPLICATION 

分 类 号：R378[医药卫生—病原生物学]