用苹果酸通透酶基因的特异性引物进行酒酒球菌快速鉴定(英文)  

Design and evaluation of specific primers targeted mleP gene for rapid identification of Oenococcus oeni

作　　者：刘延琳[1] 蒋思欣[2] 李华[1] 

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100 [2]中国科学院微生物研究所,北京100080

出　　处：《西北农林科技大学学报（自然科学版）》2006年第9期193-196,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：陕西省自然科学基金项目(2003C105);西北农林科技大学青年学术骨干支持计划(01140301)

摘　　要：以Oenococcus oeni苹果酸通透酶基因为目标基因,设计了1对特异性引物PmlepL/PmlepR进行酒酒球菌的快速鉴定研究。结果表明,直接以O.oeni的菌落为模板,通过PmlepL/PmlepR引物的PCR扩增,可得到苹果酸通透酶基因的特异性条带;用此特异性引物进行供试乳酸菌的PCR鉴定,所有O.oeni菌系均得到特异性条带,而供试的其他种类乳酸菌未扩增出目标带。引物PmlepL/PmlepR可用于O.oeni的快速PCR鉴定。Specific primers of malate permease gene （mleP） targeted were designed and evaluated for the rapid identification of Oenococcus oeni. The result indicated that specific stripes of rnleP gene could be cloned by PCR from colonies of O. oeni directly. Meanwhile,the specific stripes of rnleP gene could be cloned by PCR from all the strains of O. oeni but failed from several other species of lactic acid bacteria. The PCR assay confirmed to be specific for O. oeni species and can be used for rapid identification of O. oeni.

关 键 词：苹果酸通透酶基因 特异引物 快速鉴定 酒酒球菌 

分 类 号：Q939.97[生物学—微生物学]