血小板反应素1在STZ诱导大鼠糖尿病性视网膜病变的表达研究  

THE STUDY OF THROMBOSPONDIN-I (TSP1) EXPRESSION IN THE EARLY STAGES OF DIABETIC RETINOPATHY INDUCED BY STREPTOZOTOCIN

作　　者：王剑勇[1] 张晓明[2] 张惠燕[3] 

机构地区：[1]浙江大学医学院附属第一医院眼科,杭州310003 [2]浙江大学医学院解剖教研室,杭州310006 [3]杭州职业技术学院化工系,杭州310018

出　　处：《分子细胞生物学报》2006年第5期431-437,共7页Journal of Molecular Cell Biology

基　　金：浙江省教育厅科研基金资助项目(No.050309G20512)。~~

摘　　要：探讨TSP1表达在糖尿病视网膜病变中的作用和机制,为治疗和预防糖尿病视网膜病变提供新的实验和理论依据。用链脲佐菌素(STZ)腹腔注射建立糖尿病模型8周后,采用免疫组织化学、RT-PCR及实时荧光定量PCR法,分析TSP1在早期链尿佐菌素诱导的糖尿病SD大鼠视网膜中的表达。结果显示在早期糖尿病大鼠的视网膜表面血管、神经节细胞层、内外核层中均有明显的TSP1表达,糖尿病视网膜组TSP1 mRNA表达要高于对照组,其中实时荧光定量PCR CT值的结果显示糖尿病组TSP1 mRNA表达量较对照组要高约3．48倍,二组间差别有显著性意义(P<0．01),提示TSP1在视网膜组织中的表达与糖尿病视网膜病变的发生密不可分,TSP1表达的增加可能在糖尿病视网膜病变的发生和发展中起重要作用。Thrombospondinl （TSP1） is a known antiangiogenic factor,but its possible alteration during the early stages of diabetic retinopathy has not been explored yet. The present study sought to investigate the expression of TSP1 using a model of streptozotocin （STZ） induced diabetes in the rat. Diabetes was induced in male SD rats by an intraperitoneal injection of STZ. Age matched animals served as control. 2 months after the onset of diabetes,the expression of TSP1 mRNA was quantified in retinal tissue by quantitative real time reverse transcription-polymerase chain reaction （RT-PCR） respectively. The locations of TSP1 normal and diabetic retinas were also established by immunohistochemistry. 2 months after the onset of diabetes,retinal TSP1 concentrations were significantly increased in the diabetic rats compared with control rats. Diabetes caused the upregulation of TSP1 expression in the layers of the retina 2 months after the induction of diabetes. The upregulation of TSP1 in retinas of diabetic rats suggests a role in experimental diabetic retinopathy. Further studies should address the possible involvement of the TSP1 in the pathophysiology of diabetic retinopathy.

关 键 词：糖尿病视网膜病变 链脲佐菌素 血小板反应素-1 

分 类 号：R774.1[医药卫生—眼科]