Environmental cues associated with morphine modulate release of glutamate and γ-aminobutyric acid in ventral subiculum  

吗啡相关环境线索调节腹侧海马下托区谷氨酸和γ-氨基丁酸的释放

作　　者：康林[1,2] 戴正泽[1] 李浩洪[1] 马兰[1] 

机构地区：[1]复旦大学药理研究中心,复旦大学上海医学院药理学系,复旦大学脑科学研究所 [2]四川大学华西医院心理卫生中心,成都610041

出　　处：《Neuroscience Bulletin》2006年第5期255-260,共6页神经科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.30230130 and No.30400129);the Ministry of Science and Technology of China(No.2003CB515405,No.2005CB522406);the Program for Changjiang Scholars and Innovative Research Team of Ministry of Education of China;Shanghai Municipal Commission for Science and Technology(No.06JC14008).

摘　　要：Objective To investigate whether environmental cues associated with different properties of morphine could regulate the extracellular levels of glutamate and y-aminobutyric acid （GABA） in the hippocampal ventral subiculum, which play a critical role in the reinstatement of drug-seeking behavior induced by environmental cues. Methods Conditioning place preference （CPP） and conditioning place aversion （CPA） models were used to establish environment associated with rewarding and aversive properties of morphine respectively. Microdialysis and high performance liquid chromatography were used to measure the extracelluar level of glutamate and GABA in the ventral subiculum under these environmental cues. Results Exposure to the environmental cues associated with rewarding properties of morphine resulted in a decrease （approximately 11%） of extracellular level of GABA in ventral subiculum, and exposure to the environmental cues associated with aversive properties of morphine resulted in an increase （approximately 230%） of extracellular level of glutamate in ventral subiculum. Conclusion Environmental cues associated with different properties of morphine modulate the release of distinct neurotransmitters in the hippocampal ventral subiculum possibly through different neural circuit.目的研究吗啡相关环境线索能否调节腹侧海马下托区谷氨酸和γ-氨基丁酸的释放。方法采用条件性位置偏爱和条件性位置厌恶模型分别建立与吗啡奖赏、厌恶相关的环境线索。采用微透析和高效液相荧光检测法测定上述环境线索下大鼠腹侧海马下托区细胞外谷氨酸和γ-氨基丁酸的浓度。结果吗啡奖赏相关环境线索引起腹侧海马下托区细胞外γ-氨基丁酸浓度下降了约11%,吗啡厌恶相关的环境线索引起腹侧海马下托区细胞外的谷氨酸浓度增加了约230%。结论吗啡相关的环境线索可能通过不同的神经环路调节腹侧海马下托区神经递质的释放。

关 键 词：conditioning place preference conditioning place aversion ventral subiculum MICRODIALYSIS γ-aminobutyric acid GLUTAMATE 

分 类 号：R96[医药卫生—药理学]