调控细胞通透性以提高透明质酸的产量和分子量  

Study on the controlling infiltrates of cells to increase hyaluronic acid production and its molecule

作　　者：汪江波[1] 薛海燕[1] 邹玉玲[1] 刘宏 

机构地区：[1]湖北工业大学生物工程学院,湖北武汉430068 [2]金龙泉啤酒孝感有限公司,湖北孝感431200

出　　处：《华西药学杂志》2006年第5期465-467,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的诱变选育甘油缺陷型菌株,调控细胞膜的通透性以提高透明质酸的产量和分子量。方法经诱变马疫链球菌,选育甘油缺陷型菌株,添加甘油调节细胞膜的通透性,添加溶菌酶调控细胞壁的通透性。结果当甘油的添加量为0.0025%,溶菌酶先后两次的添加量为2.8×104、4.8×104u.L-1时,透明质酸的产量可从4.70 g.L-1增加到6.07 g.L-1,分子量从1.01×106增加到1.7×106。结论所选甘油缺陷型菌株,可调控细胞的通透性,透明质酸的产量和分子量都可达到较高水平。OBJECTIVE To select a strain without the production of L -- glycerin - 3 - calcium phosphate. METHODS Streptococcus equin was treated with NTG, and cultured with adding L - glycerin - 3 - calcium phosphate and lysozyme to control cell wall infil- trates and cell membrane infiltrates, respectivelly. RESULTS By adding 0. 0025% L - glycerin - 3 - calcium phosphate and 2. 8 × 10^4,4. 8 ×10^4 U·L^-1 lysozyme, the production of hyaluronic acid increases from 4.7g·L^-1 to 6,0 g· L^-1 with increased molecule weight from 1.01× 10^6 to 1.7 ×10^6. CONCLUSION A strain without the production of L - glycerin - 3 - calcium phosphate is firstly selected. Methed of controlling cell infiltrates to increase molecule and production of hyaluronic acid is different from other research, which produce higher production and molecule.

关 键 词：透明质酸 通透性 马疫链球菌 分子量 甘油缺陷性菌株 

分 类 号：TQ920[轻工技术与工程—发酵工程]