非小细胞肺癌线粒体基因组大片段缺失突变研究  

Study on 4977 bp deletion mutation of mitochondrial DNA in non-small lung cancer

作　　者：戴纪刚[1] 肖颖彬[1] 闵家新[2] 张国强[2] 向明章[2] 吴秋平[2] 姚珂[2] 周人杰[2] 余祖斌[2] 

机构地区：[1]第三军医大学新桥医院心血管外科,重庆400037 [2]第三军医大学新桥医院胸外科,重庆400037

出　　处：《第三军医大学学报》2006年第21期2123-2126,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(39900173);重庆市自然科学基金资助项目(2005)~~

摘　　要：目的明确mtDNA4977bp大片段缺失在肺癌组织、癌旁正常组织及非癌患者肺组织中的分布频率,探讨mtDNA4977bp大片段缺失与肿瘤的关系。方法采用长距离PCR方法对37例肺癌组织和相应的癌旁正常组织、20例非癌患者正常肺组织mtDNA4977bp缺失进行了检测。结果肺癌组织、癌旁正常肺组织及非癌患者正常肺组织中均检测出存在有线粒体DNA4977bp片段缺失。37例肺癌组织标本中有20例(54.1%)检测到4977bp缺失,37例相应的癌旁肺组织22例(59.5%)有缺失,其中8例在肺癌组织中未显示而却在癌旁正常组织检测到缺失,20例非癌患者正常肺组织中也有6例出现4977bp片段缺失。线粒体DNA4977bp缺失与年龄、吸烟因素有关。结论线粒体DNA4977bp缺失并非肺癌特异性突变,在癌变过程中不太可能起重要的作用,而可能仅仅是肿瘤发生发展过程中环境和遗传因素作用的反映。Objective To study the 4 977 bp deletion of mitochondrial DNA in lung cancer, paraneoplastic tissue and normal lung tissue from non-lung cancer subjects and its significance in the development of cancer. Methods Lung cancer tissues and paraneoplastic tissues from 37 non-small lung cancer patients, and normal lung tissues from 20 patients without lung cancer were analyzed by long PCR technique. Results Mitochondrial DNA 4 977 bp deletion was detected in 54.1% （20/37） of lung cancer tissues, 59.5% （22/37） of paraneoplastic tissues and 30.0% （6/30） of normal lung tissues. The correlation between 4 977 bp deletion and age, smoking was present in our data. Conclusion Mitochondrial DNA 4 977 bp deletion, which may reflect the environmental and genetic influences during tumor progression, is not specific to lung cancer and unlikely to play an important role in carcinogenesis.

关 键 词：线粒体DNA 肺癌 缺失 突变 

分 类 号：R394.2[医药卫生—医学遗传学] R730.23[医药卫生—基础医学]