改良热酚水法制备大肠杆菌O157:H7脂多糖抗原的研究  

Preparation of Specific Lipopolysaccharide Antigen of Enterohemorrhagic Escherichia coli O157:H7 with Advanced Hot Phenol-water Method

作　　者：宋宏新[1] 刘晓阳[1] 李宏[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西咸阳712081

出　　处：《食品科学》2006年第10期273-275,共3页Food Science

摘　　要：采用热酚水法提取肠出血性大肠杆菌(EHEC)O157:H7的脂多糖(LPS),经浓缩酶解,离心分离得纯化LPS,酸解制得O-特异性侧链(O-PS),能有效去除核酸及杂蛋白,抗原具有鲎试剂凝集活性及对产蛋鸡有较强的免疫原性,建立了一种改良热酚水法提取制备O157:H7特异性脂多糖抗原的方法。The LPS antigen was prepared from EHEC O157：H7 strain 933 with hot phenol water method and purified by enzyme hydrolyzing with DNase and RNase, and then hydrolyzed by 1.5% acetic acid for getting OPS. The O157：H7 LPS antigen has high purity specificity, has activity of agglutinating limulus amebocyte lysate and high immunoreactivity for chicken. They could be used to produce specific anti-LPS antibody.

关 键 词：EHEC O157∶H7 热酚水法 脂多糖抗原制备 

分 类 号：Q939.91[生物学—微生物学]