固相萃取和高效液相色谱法测定甜茶中的黄酮类物质  

Study on Flavonoid Assay in Lithhocarpus polgstachrch Rehd with Solid Phase Extraction and HPLC

作　　者：杨亚玲[1] 赵红芳[1] 苏毅[1] 赵榆林[1] 周强[1] 

机构地区：[1]昆明理工大学生物与化学工程学院,云南昆明650224

出　　处：《食品科学》2006年第9期197-199,共3页Food Science

摘　　要：研究了用固相萃取预分离,高效液相色谱法测定甜茶中黄酮类物质的方法。甜茶中的黄酮用80%乙醇溶液回流提取,提取液用WatersSep-Pak-C18固相萃取小柱预分离脱脂,以WatersNova-Pak-C18(3.9×150mm,5μm)色谱柱为固定相,以0.05mol/L磷酸二氢钾缓冲溶液和甲醇的比例为40:60(V/V)为流动相,在该色谱条件下,甜茶中主要的黄酮成分均达到基线分离;用紫外二极管矩阵检测器在360nm波长处检测,并作了色谱峰纯度分辨。方法标准回收率为97%～103%,相对标准偏差为0.87%～2.2%。A high performance liquid chromatography （HPLC） method for the flavonoid assay in Lithhocarpus polgstachrch Rehd was studied. The flavonoid was extracted from the sample with 80% of ethanol by heated reflux. Then the extract was degreased by solid phase extraction with Sep-Pak-C18 cartridge. The flavonoid was separated on a Nova-Pak-C18 chromatogram column （3.9 ×150mm, 5 μm）, with methanol and 0.05mol/L potassium dihydrogen phosphate buffer solution as mobile phase at 60：40 （V/V）, and monitored with the photodiode array detector at 360nm. The recoveries of the flavonoid are 97%-103%, and relative standard deviations are 0.87% -2.2%.

关 键 词：高效液相色谱 固相萃取 甜茶 黄酮 

分 类 号：O657.7[理学—分析化学]