穿山龙薯蓣皂苷酶解产物的分离纯化  

Separation and purification of dioscin hydrolysate

作　　者：刘冰[1] 王元好[1] 朱宏丽[1] 鱼红闪[1] 金凤燮[1] 

机构地区：[1]大连轻工业学院生物与食品工程学院,辽宁大连116034

出　　处：《大连轻工业学院学报》2006年第3期161-163,共3页Journal of Dalian Institute of Light Industry

基　　金：国家自然科学基金资助项目(303717442047601730470055)

摘　　要：用柱层析法分离纯化穿山龙薯蓣皂苷的酶解产物,硅胶柱中的流动相为氯仿与甲醇的不同比例,经硅胶柱分离得到薯蓣皂苷酶解产物的两种单体,为产物Ⅰ和产物Ⅱ,用薄层层析法和高效液相色谱法检测,得到产物Ⅰ为3-(O)-(-βD-吡喃葡萄糖基)-薯蓣皂苷元,得率为17.85%,纯度为69.41%。同时得到产物Ⅱ,得率为1.64%。产物2为反应副产物,其结构、性质及药理作用有待于进一步研究。Two kinds of dioscin hydrolysates were separated and purified by silica gel column gradient elution with chloroform and methanol as solvent. The hydrolysate I was identified to be 3-（O）-（β-D- glucopyranoside）-diosgenin by TLC and HPLC. The yield of the purified dioscin from enzyme reaction was 17. 85%. The purity of 3-（O） -（β-D-glucopyranoside） -diosgenin was detected to be about 69. 4190 . The hydrolysate Ⅱ is an outgrowth with 1.64% of yield. Its structure, property, pharmacology functions are yet to be investigated.

关 键 词：薯蓣皂苷 酶解产物 分离 纯化 

分 类 号：Q946.5[生物学—植物学]