原发性肝癌患者乙型肝炎病毒C基因启动子变异与HBV DNA关系的探讨  

Relationship between HBV c gene promoter gene mutation and serum HBV DNA contents in HCC

作　　者：玉艳红[1] 黄力毅[1] 宣伟军[2] 庞辉[3] 

机构地区：[1]广西医科大学第一附属医院,南宁530021 [2]广西中医学院,南宁530001 [3]广西医科大学生理学教研室,南宁530021

出　　处：《应用预防医学》2006年第5期257-259,共3页Applied Preventive Medicine

基　　金：广西科学基金项目(桂科自0339050);广西卫生厅课题(桂卫Z2004127)

摘　　要：目的研究原发性肝癌患者乙型肝炎病毒C基因启动子变异(HBVBCP)与HBVDNA关系。方法(1)采用PCR微板核酸杂交结合ELISA检测显示技术,对患者血清进行检测HBVBCP区核苷酸(nt)1762碱基A→T和1764G→A联合突变。(2)采用PCR结合荧光探针检测技术,检测患者血清HBVDNA含量。结果(1)HBVBCP变异在原发性肝癌组的阳性率为70.0%(14/20),显著高于慢性肝病组的阳性率37.8%(59/156)(P=0.008)。(2)原发性肝癌组的血清HBVDNA含量(107.6414±1.3398拷贝/ml)明显高于慢性肝病组的HBVDNA含量(106.7672±1.7669拷贝/ml)(P=0.034)。结论HBVBCP变异与原发性肝癌关系密切,且血清HBVDNA复制水平在原发性肝癌的发生中可能也起到主要的作用。Objective In order to investigate the relationship between hepatitis B virus （HBV） basic core promoter （BCP） mutation and serum HBV DNA contents in hepatocellular carcinoma （HCC） patients. Methods （ 1 ）The A to T mutation at nucleotide 1762 and G to A mutation at nucleotide 1764 were determined by the method of polymerase chain reaction （PCR） microplate hybridization ELISA in these patients. （2） Serum HBV DNA of these patients were detected by fluorescence PCR. Results （1） The positive rate of HCC was significantly higher than that of chronic liver disease（ P = 0.008） . （2） The quantity of HBV DNA in HCC group （10^7.6414±1.3398 copy/ ml） was significantly higher than that in chronic liver disease group （10^6.7672±1.7669 copy/ ml） （P = 0.034） . Conclusion HCC is correlated with HBV BCPmutants. There was a high positive rate of HBV BCP mutants in the HCC.

关 键 词：原发性肝癌 乙型肝炎病毒核心基因启动子 基因变异 HBV DNA 

分 类 号：R735.702[医药卫生—肿瘤]