高效液相色谱法测定豆粕中异黄酮含量的研究  

Study on determination, isoflavine from soy residue by HPLC

作　　者：高荣海[1] 李长彪[1] 孟宪文[1] 赵秀红[1] 刘长江[1] 

机构地区：[1]沈阳农业大学食品学院,沈阳110161

出　　处：《食品科技》2006年第10期234-237,共4页Food Science and Technology

基　　金：辽宁省科技厅科技攻关项目(2004205001)

摘　　要：介绍了高效液相色谱同时测定豆粕中4种大豆异黄酮的方法。确定出色谱条件为:色谱柱HypersilODS2C18柱(250mm×4.6mmID,5μm),流动相甲醇∶水=40∶60,柱温3O℃,流速1.0mL/min,检测波长260nm。大豆苷、染料木苷、大豆苷元、染料木素在0.21～8.56μg/mL范围内浓度与峰面积具有很好的线性关系,相关系数r为0.999155～0.999991。该测定方法的回收率大于99%,变异系数小于3%。A method for simultaneous determination of four soybean isoflavone from soy residue by high perform -ance liquid chromatography was described. The operating conditions were：The column was Hypersil ODS2 C18 （250mm×4.6mmID,5μm）, the mobile phase was MeOH：H2O=40：60, the temperature of column was 30%, the flow rate was 1.0 mL/min. The detection wavelength was 260nm. The liner range of daidzin, genistin, daidzein and genistein was 0.21-8.56tJg/mL with a correlatione coeff-icient of 0.999155-0.999991. The average recovery rate was more than 99% with RSD less than 3%.

关 键 词：大豆异黄酮 高效液相色谱法 测定 

分 类 号：O657.72[理学—分析化学]