PCR检测沙门氏菌invA基因的灵敏度  

Sensitivity of PCR detection for the inva gene in salmonella

作　　者：江树勋[1] 吴圣静[1] 李寿崧[1] 饶静静[1] 江云[1] 

机构地区：[1]福建出入境检验检疫局检验检疫技术中心,福州350001

出　　处：《食品科技》2006年第10期251-253,共3页Food Science and Technology

基　　金：福建省科技厅重点项目(2004Y046)

摘　　要：沙门氏菌是一种重要的肠道致病菌,其invA基因编码吸附和侵袭上皮细胞表面蛋白,应用PCR扩增invA基因检测致病性沙门氏菌,并测定其PCR检测灵敏度。结果显示,扩增出的284bp大小的序列为目的条带,实验可检出的最小灵敏度为11.542pg。Salmonella is one of the important intestinal pathogenic bacteria,its invA Gene codes the protein which absorbs and attacks the epithelium,This paper studies the detection of the invA gene by PCR method, and its detection sensitivity.The results shows that an expected 284bp band is successfully amplified,and the lowest limit of the sensitivity is 11.542pg.

关 键 词：沙门氏菌 PCR 灵敏度 

分 类 号：TS207.3[轻工技术与工程—食品科学]