乳腺癌组织中ERβmRNA和VEGFmRNA的表达及两者的关系  

The relationship between the expression of estrogen receptor beta mRNA and vascular endothelial growth factor mRNA in human breast cancer

作　　者：吴唯[1] 吕新生[1] 唐中华[2] 李小荣[3] 陈道谨[3] 

机构地区：[1]中南大学湘雅医院普通外科,湖南长沙410008 [2]中南大学湘雅二医院普通外科,湖南长沙410011 [3]中南大学湘雅三医院普外二科,湖南长沙410013

出　　处：《中国普通外科杂志》2006年第10期741-744,共4页China Journal of General Surgery

摘　　要：目的探讨雌激素受体亚型β(ERβ)和血管内皮细胞生长因子(VEGF)在乳腺癌组织中的表达及两者之间的关系。方法采用半定量逆转录聚合酶链式反应(RT-PCR)检测3 5例乳腺癌组织中ERβmRNA和VEGFmRNA的表达,分析两者表达的相关性及其与肿瘤病理参数的关系。结果ERβmRNA表达水平与VEGF1 2 1和VEGF1 6 5mRNA的表达水平呈正相关(r=0.7 8 5和0.6 4 1,均P=0.0 0 0)。VEGF1 2 1和VEGF 1 6 5mRNA在有淋巴结转移的乳腺癌组织中表达水平明显高于无淋巴结转移者(P=0.0 3 3,0.0 0 4)。结论乳腺肿瘤血管生成可能受ERβ的影响,而乳腺癌组织中VEGFmRNA的表达水平高者可能易于发生淋巴结转移。Objective To study the expression of estrogen receptor beta mRNA （ ERβ mRNA ） and vascular endothelial growth factor mRNA （ VEGF mRNA ） in breast cancer （ BC ） and the interrelationship of their expression. Methods Semi-quantitive reverse transcriptase polymerase chain reaction was conducted to dectect the expression of ERβ mRNA and VEGF mRNA in 35 BC samples. The correlation between the expression of ERβ mRNA and VEGF mRNA was analyzed, and the relationship between their expression and clinicalpathological parameters was also analyzed. Results The expression of VEGF 121, VEGF 165 mRNA in BC with lymph node metastasis was significantly higher than that in BC without lymph node metastasis （ P = 0. 033,0. 004 ）. The expression of ERβ mRNA was well correlated with the expression of VEGF121, 165 mRNA in BC patients （ all p = 0. 000 ） . Conclusions ERβ might modulate the expression of VEGF mRNA in BC. The expression of VEGF mRNA with high level in BC patient can indicate that lymph node metastasis might occur.

关 键 词：乳腺肿瘤/病理学 受体 雌激素 血管内皮细胞生长因子 

分 类 号：R737.9[医药卫生—肿瘤] Q51[医药卫生—临床医学]