非水相脂肪酶催化阿魏酸双甘酯的合成  

Synthesis of feruloylated diacylglycerols by lipase-catalyzed tansesterification in non-aqueous medium

作　　者：辛嘉英[1] 郑妍[1] 吴小梅[2] 夏春谷[2] 李树本[2] 

机构地区：[1]哈尔滨商业大学生物工程系,黑龙江哈尔滨150076 [2]中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室,甘肃兰州730000

出　　处：《现代化工》2006年第10期52-54,56,共4页Modern Chemical Industry

基　　金：教育部新世纪优秀人才支持计划(NCET-05-0358);黑龙江省自然科学基金(B200403);黑龙江省教育厅基金(160134);哈尔滨市科学基金(2004AFXXJ031)

摘　　要：在有机相和无溶剂反应体系中进行不同来源的脂肪酶催化阿魏酸与油酸甘油酯转酯反应的比较研究。通过筛选发现固定化脂肪酶Novozym435因其独特的催化专一性减少了副产物,提高了目标产物的产率。同时研究了不同反应介质对转酯反应的影响,结果表明在无溶剂反应体系中,Novozym435脂肪酶催化阿魏酸乙酯与油酸甘油酯的转酯反应目标产物得率较高,与甲苯作为反应介质的体系相比酶的操作稳定性得到了明显提高。Feruloylated diacylglycerols was synthesized by various lipases in both organic phase and solvent-free system, and the transesterification reactions in those two different solvent systems were compared. It is found that the high yield and less by-products in the reaction catalyzed by Novozym435 made it a more appropriate catalyst for the traneseterification reaction. Comparing with the organic reaction system, the solvent-free system showed its unique predominance as a reaction medium. The Novozym435 lipase catalyzed reaction in a solvent-free system was repeated fifth times with the recycling of enzyme and has good operational stability.

关 键 词：阿魏酸 阿魏酸双甘酯 脂肪酶 转酯反应 

分 类 号：Q559[生物学—生物化学] TQ225.24[化学工程—有机化工]