葡萄汁对人前列腺癌PC-3细胞增殖和凋亡的影响  

EFFECTS OF GRAPE JUICE ON PROLIFERATION AND APOPTOSIS OF HUMAN PROSTATE CARCINOMA PC-3 CELLS

作　　者：罗琼[1] 庞雅琴[2] 杨明亮[1] 阎俊[1] 王丽红[1] 崔晓燕[1] 

机构地区：[1]武汉大学公共卫生学院卫生学教研室,武汉430071 [2]右江民族医学院,百色533000

出　　处：《营养学报》2006年第5期418-422,共5页Acta Nutrimenta Sinica

基　　金：湖北省科技厅重点攻关课题(No.2003GX1B135)

摘　　要：目的:探讨葡萄汁对人前列腺癌PC-3细胞生长抑制的作用及其机制。方法:白藜芦醇及不同浓度葡萄汁作用于人前列腺癌PC-3细胞48h;采用生长曲线及噻唑蓝(MTT)法检测葡萄汁、白藜芦醇对人前列腺癌PC-3细胞的生长抑制作用,通过原位(缺口)末端标记法(TUNEL)观察凋亡细胞的形态结构变化,流式细胞仪检测细胞凋亡峰。结果:葡萄汁、白藜芦醇能显著抑制人前列腺癌PC-3细胞生长;TUNEL方法检测呈阳性;流式细胞仪分析图上出现典型的凋亡细胞峰。以上各指标中,葡萄汁随着浓度增大作用加强;低剂量组的作用比白藜芦醇组差,中、高剂量组的白藜芦醇浓度低,但作用比白藜芦醇组强。结论:葡萄汁对抗人前列腺癌PC-3细胞的作用,除白藜芦醇外,可能还存在其他抗癌成分的作用。葡萄汁、白藜芦醇对PC-3细胞生长抑制作用的机制可能与其诱导人前列腺癌PC-3细胞凋亡有关。Objectives： To study the inhibitory effects of grape juice on human prostate carcinoma PC-3 cells and its possible mechanism. Method： PC-3 cells were treated with grape juice in different concentration（16, 32, 64μl/ml） and resveratrol for 48 h; and the proliferation of PC -3 cells were measured by growth curve and MTT assay. TUNEL was used to observe the morphology of apoptotic cells and flow cytometry to analyze the PC-3 apoptosis. Results： Resveratrol and grape juice could markedly inhibit the proliferation of PC-3 cells, and TUNEL positive cells were detectable. The percentages of apoptotic cells were 6.0%, 18.5%, 30.0%, 12.7% in experimental groups and resveratrol group respectively. The effect on PC-3 cells was enhanced with increasing amount of grape juice. The effect of low dose group was weaker than that of resveratrol group, but medium and high dose groups were stronger. Conclusion： The anti-prostate cancer effect of grape juice is not only related to resveratrol contained but also to synergism with other components, and its mechanism might be the induction of PC-3 cells apoptosis.

关 键 词：葡萄汁 白藜芦醇 人前列腺癌PC-3细胞 

分 类 号：R737.25[医药卫生—肿瘤]