燕麦β-葡聚糖对小鼠结肠菌群及其功能的影响  

EFFECT OF OAT β-GLUCAN ON COLON FLORA AND ITS FUNCTION IN MICE

作　　者：申瑞玲[1] 王章存[2] 董吉林[1] 姚惠源 

机构地区：[1]山西农业大学,太谷030801 [2]郑州轻工业学院,郑州450002 [3]江南大学,无锡214036

出　　处：《营养学报》2006年第5期430-433,共4页Acta Nutrimenta Sinica

基　　金：山西省自然科学基金(No.20031071)

摘　　要：目的:研究燕麦β-葡聚糖对小鼠结肠菌群及其功能的影响。方法:体重23～25g的健康昆明种小鼠126只,分成7组,每组18只,实验组(1～6组)分别以高低剂量0.5g和0.25g/(㎏bw·d)灌胃给予燕麦β-葡聚糖OG2600、OG340和OG5,对照组给予相同体积的生理盐水,灌胃期28d。分别于实验14、28和35d(停止灌胃后1w)分析各组小鼠结肠双歧杆菌、乳酸杆菌和大肠杆菌的变化;并在实验28d用气相色谱法测定小鼠结肠中短链脂肪酸的含量;用流式细胞仪分析结肠粘膜上皮细胞变化。结果:与对照组比,随着灌胃时间的延长,各实验组均可使小鼠肠道和粪便中的双歧杆菌和乳酸杆菌增殖,使大肠杆菌数量减少,作用效果与分子大小和剂量有关;第2、4、6组小鼠结肠内容物的乙酸、丙酸、丁酸的量显著高于对照组(P<0.05);第6组细胞增殖指数和对照组有显著差异(P<0.05)。结论:燕麦β-葡聚糖具有调节小鼠肠道菌群的作用;在体内容易发酵产生较高比例的短链脂肪酸,能促进结肠粘膜上皮细胞的增殖。Objective： To investigate the effect of oat β-glucan on colon flora and its function in mice. Method：126 Kunming mice （23-25g） were divided into seven groups （18 mice in each）. The group 1 and 2, 3 and 4,5 and 6 were respectively administered oat β-glucan （OG2600 , OG340 and OG5） at dose of 0.25 and 0.5g/ （kg bw · d） daily for 28 d. The control group received the equal volume of normal saline. Bifidobacterium, Lactobacillus and Enterobacillus were monitored in colon after oral administration of oat β-glucan at 14 d, 28 d and 35 d （after terminating oat β-glucan admimistration for 1 w）. The contents of short chain fatty acids （SCFA） in colon were analyzed by GC and cell proliferation in colonic mucosa epithelium by FCM in each group. ResuIts： The numbers of Bifidobacterium, Lactobacillus compared with the control were significantly increased and Enterobacillus strains were decreased in experimental groups. Oat β-glucan had the function of regulating intestinal flora and the effect was related to molecular weight and dosage. The contents of acetate, propionate, butyrate were higher in the group 2, 4 and 6 compared with the control （P〈0.05）in colon. The proliferation of the colonic mucosa cells in group 6 was significantly higher than that in the control group （P〈0.05）. Conclusion： Oat β-glucan can regulate colon flora and enhance the proliferation of the colonic mucosa cells.

关 键 词：燕麦 Β-葡聚糖 结肠菌群 短链脂肪酸 

分 类 号：R151[医药卫生—营养与食品卫生学]