实验性高血压大鼠局灶性脑缺血再灌注后肾上腺髓质素及其mRNA的表达  

Expression of Adrenomedullin and Its mRNA in the Experimental Hypertension Rats After Focal Cerebral Ischemia and Reperfusion

作　　者：毕国荣[1] 张贺敏[1] 周慧杰[1] 海虹[1] 白丽娟[1] 张辉[1] 方秀斌[2] 

机构地区：[1]中国医科大学第二临床学院神经内科,辽宁省沈阳市110004 [2]中国医科大学基础医学院神经生物学教研室,辽宁省沈阳市110004

出　　处：《中国动脉硬化杂志》2006年第8期653-656,共4页Chinese Journal of Arteriosclerosis

基　　金：辽宁省自然科学基金(编号20032058)资助

摘　　要：目的探讨肾上腺髓质素与脑缺血再灌注损伤及高血压的关系。方法应用改良Kellen的方法制备实验性高血压大鼠,采用栓线法制成大鼠大脑中动脉闭塞模型,阻断血流2 h后进行再灌注。免疫组织化学法检测高血压大鼠与非高血压大鼠局灶性脑缺血再灌注后肾上腺髓质素阳性细胞百分率并进行比较,逆转录聚合酶链反应检测大鼠局灶性脑缺血再灌注后脑组织肾上腺髓质素mRNA表达的情况。结果正常大鼠脑内有肾上腺髓质素mRNA表达,假手术后肾上腺髓质素mRNA表达略有增加,但与正常对照组相比差异无显著性(P>0.05);大鼠脑缺血再灌注后肾上腺髓质素mRNA过表达,与正常对照组及假手术组相比差异有显著性(均P<0.05);大鼠脑缺血再灌注后,缺血侧及缺血对侧肾上腺髓质素mRNA均有高表达,但以缺血侧最显著(P<0.05)。正常对照组和假手术组大鼠大脑组织中均有肾上腺髓质素阳性细胞表达,肾上腺髓质素阳性细胞百分率分别为2.87%±0.78%和2.47%±0.59%(P>0.05)。缺血再灌注非高血压组肾上腺髓质素阳性细胞高表达,以缺血侧为明显,阳性细胞百分率为59.42%±3.71%,缺血对侧亦明显,阳性细胞百分率为36.87%±5.28%,两者相比差异有显著性(P<0.05),与假手术组及正常对照组相比差异也有显著性(P<0.05)。比较高血压组与非高血压组肾上腺髓质素阳性细胞百分率发现,高血压组肾上腺髓质素阳性细胞百分率(缺血侧为78.60%±4.82%,缺血对侧为57.52%±5.22%)明显高于非高血压组(P<0.05)。结论脑缺血再灌注后肾上腺髓质素及其mRNA高表达。高血压组肾上腺髓质素的高表达更显著。肾上腺髓质素与脑缺血再灌注损伤相关,与高血压血管内皮损伤有关。Aim To investigate the relationship of adrenomedullin and its mRNA with the focal cerebral ischemia/reperfusion injury and hypertension in rats. Methods The experimental hypertension rats were prepared by the advanced Kellen＇s methods. The models of middle cerebral artery occlusion in rats were constructed by the line-block-method, and after ischemia for 2 h they were reperfused. By using the immtmohistochemical method, the percentage of adrenomedullin positive cells both in hypertension group and nonhypertension group after focal cerebral ischemia/reperfusion was determined and compared, and the expression of adrenomedullin mRNA in the brain tissue after focal cerebral ischemia/reperfusion in rats was monitored by the RT-PCR analysis. Results Adrenomedullin mRNA expressed in the normal rats＇ brains, and it increased slightly in the sham operated group, but it had no significant difference from the normal group（ P 〉 0.05 ）. The over-expression of ADM mRNA existed after cerebral ischemia/reperfusion in rats, which had significant difference with the normal group and the sham operated group（ P 〈 0.05 both）. After focal cerebral ischemia/reperfusion in rats, adrenomedullin mRNA in not only the ipsilateral but also the contralateral side of the ischemic area expressed high, especially in the ischemic area （ P 〈 0.05 ）. The adrenomedullin positive cells expressed in the brain tissue of the rats in the normal group and the sham operated group, and their percentages were 2.87% ± 0.78 % versus 2.47 % ± 0.59%, both of which had no significant difference （ P 〉 0.05 ）. The adrenomedullin positive cells expressed high in the non- hypertension group after ischemia/reperfusion, especially in the ischemic side, whose percentage of the positive cells was 59.42% ± 3.71%, and the centralateral side was also significant, whose percentage of the positive cells was 36.87% ± 5.28%, and the differences between them was significant（ P 〈 0.05）, which was also significandy different from the sham oper

关 键 词：神经病学 肾上腺髓质素 实验性高血压大鼠 脑缺血再灌注 免疫组织化学 逆转录聚合酶链反应 

分 类 号：R743[医药卫生—神经病学与精神病学]