食品中黄曲霉毒素B_1单克隆抗体酶联免疫测定方法的建立及初步应用  

Establishment of ELISA for the determination of aflatoxin B_1 in food and its preliminary application

作　　者：路戈[1] 计融[2] 刘春霞[1] 

机构地区：[1]北京市营养源研究所,北京100054 [2]卫生部食品卫生监督检验所

出　　处：《卫生研究》1996年第3期162-165,共4页Journal of Hygiene Research

基　　金：北京市自然科学基金

摘　　要：用抗 AFB_1的单克隆抗体 AFB_1-2H8,建立了检测食品(玉米、花生、小麦、大米、植物油)中 AFB_1的 ELISA 间接竞争法。该方法最低检出浓度为0.01ng/g,敏感范围为0.2～50ng/g,平均回收率为83.0%～110.3%,精密度为2.0%～24.3%。用该法测定了分布于淮河以北地区的145份样品,并对25份植物油样品用 TLC 和 ELISA 两种方法进行了对比测定。Indirect competitive ELISA for the determination of AFB_1 in food (including corn,peanut,wheat,rice and plant oil) was established with an anti-AFB_1 McAb,designated as AFB_1-2H8.The sensitive range of the method was 0.2～50ng/g and the minimum detectable concentration of AFB_1 was 0.01ng/g.The mean recovery rates and CV(%) from sample spiked with 1～100ng/g of AFB_1 were 83.0%～110.3% and 2.0% ～24.3% respectively.A total of 146 samples collected from north area of Huaihe River in China were ana- lyzed and the detection compared ELISA with TLC for AFB_1 in 25 plant oil samples were also completed.

关 键 词：黄曲霉毒素B1 ELISA 

分 类 号：R155.5[医药卫生—营养与食品卫生学]