The expression of TRPA1 mRNA in the rat brain  

作　　者：Peng Du Shua Li Jinyu Zheng Zhi-yuan Yu Minjie Xie Wei Wang 

机构地区：[1]Department of Neurology,Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China [2]Shanghai Institute of Neuroscience, Shanghai 200030,China [3]Department of Pathology,Drum Tower Hospital, Nanjing University, Nanjing210008 ,China

出　　处：《Journal of Nanjing Medical University》2006年第4期225-228,共4页南京医科大学学报（英文版）

基　　金：Major Project of National Scientific Foundation Committee(NSFC30230140,30400142) and Program for New Century Excel-lent Talentsin University(NCET-04-0695)

摘　　要：Objective： To investigate the distribution of TRPA1 （one kind of the TRP-like ion channel family） channel in the hippocampus and cerebral cortex of rat. Methods： RT-PCR was used to amplify the fragment of TRPA1 in the DRG （dorsal root ganglion）, hippocampus and cerebral cortex of adult SD rat. In situ hybridization staining was used to show the distribution of TRPA1 mRNA in the hippocampus and cerebral cortex of adult rat brain. Results： Both RT-PCR and in situ hybridization staining showed that TRPA1 mRNA was expressed in hippocampus and cerebral cortex of the adult rat brain. Conclusion： Our results suggest that there is expression of TRPA1 mRNA both in the hippocampus and cerebral cortex of the adult rat brain.Objective： To investigate the distribution of TRPA1 （one kind of the TRP-like ion channel family） channel in the hippocampus and cerebral cortex of rat. Methods： RT-PCR was used to amplify the fragment of TRPA1 in the DRG （dorsal root ganglion）, hippocampus and cerebral cortex of adult SD rat. In situ hybridization staining was used to show the distribution of TRPA1 mRNA in the hippocampus and cerebral cortex of adult rat brain. Results： Both RT-PCR and in situ hybridization staining showed that TRPA1 mRNA was expressed in hippocampus and cerebral cortex of the adult rat brain. Conclusion： Our results suggest that there is expression of TRPA1 mRNA both in the hippocampus and cerebral cortex of the adult rat brain.

关 键 词：TRPA1 RT-PCR in situ hybridization HIPPOCAMPUS cerebral cortex RAT 

分 类 号：R651.1[医药卫生—外科学]