一株高产谷氨酰胺合成酶的LNU 0165菌的鉴定  

Identification on Glutamine Synthetase High-Producing Strain LNU 0165

作　　者：魏杰[1] 李昱[1] 辛如[1] 刘宏生[1] 

机构地区：[1]辽宁大学生命科学系,辽宁沈阳110036

出　　处：《辽宁大学学报（自然科学版）》2006年第4期349-352,共4页Journal of Liaoning University：Natural Sciences Edition

基　　金：辽宁省科技攻关项目(2004205004)

摘　　要：谷氨酰胺合成酶是应用广泛的生物酶类,以LNU 0165为实验出发菌株,对不同pH、温度条件下产谷氨酰胺合成酶的最佳发酵条件进行了研究,通过测定其谷氨酰胺合成酶酶活力,确定LNU 0165为高产菌株.从分子水平上对该菌株进行16S rRNA序列分析及同源性比较,以及生理生化的鉴定.根据LNU0165菌株16S rRNA与GenBank数据库中Arthrobacter globiform is的16S rRNA的序列具有99%的同源性,确定LNU 0165为球形节杆菌(Arthrobacter globiform is).Glutamine Synthetase （GS） is a biological enzyme which has been widely used. The optimum fermentation conditions which produce glutamine synthetase in different temperatures and pH about LNU 0165 was studied. LNU 0165 is a high - producing strain by determining activity of GS. Moreover its phylogenetic tree was studied genomically by its 16S rRNA sequence and homology. According to 99% of sequence of homology of 16S rRNA and database in GenBank, finally, LNU 0165 strain was identified as Arthrobacter globiformis.

关 键 词：谷氨酰胺合成酶 节杆菌 16S RRNA 鉴定 

分 类 号：TQ92[轻工技术与工程—发酵工程]