非霍奇金淋巴瘤BCL-XL基因表达及突变的研究  

BCL-XL Expression and Mutation in Non-Hodgkin′s Lymphoma

作　　者：刘元华[1] Christophe Leboeuf 金晓龙[3] 肖家诚[3] Anne Janin 陈赛娟[1] 赵维莅[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院 [2]法国国立卫生研究所/法国巴黎第七大学 [3]上海交通大学医学院附属瑞金医院病理科,上海200025

出　　处：《中国实验血液学杂志》2006年第5期903-907,共5页Journal of Experimental Hematology

基　　金：上海市科委国际合作项目(编号44107025);中法先进研究计划(编号B05-02);上海市科委启明星计划(编号05QMX1429)

摘　　要：本研究探讨78例非霍奇金淋巴瘤BCL-XL表达和突变的发生率及其临床意义。应用激光微切割技术从淋巴结组织中特异地分离淋巴瘤细胞,用实时定量RT-PCR法检测淋巴瘤组织和淋巴瘤细胞中BCL-XL的表达,PCR直接测序法检测BCL-XL的突变情况。结果表明:与淋巴结反应性增生(15例)相比,滤泡性淋巴瘤(30例)组织和微切割的淋巴瘤细胞均高表达BCL-XL(P值分别为0.0064和<0.0001),而T细胞淋巴瘤(24例)和弥漫性大B细胞淋巴瘤(24例)中BCL-XL表达无明显升高。在滤泡性淋巴瘤中,BCL-XL高表达的患者常伴多个淋巴结外器官累及(P=0.0004),血清乳酸脱氢酶水平升高(P=0.0019),国际预后指数分组多为高危组(P=0.0013),患者总生存期短(P=0.0451)。突变检测发现1例滤泡性淋巴瘤BCL-XL的同义突变(密码子109ACA→ACC)。结论:BCL-XL表达与滤泡性淋巴瘤的疾病进展和患者预后密切相关。The study was aimed to investigate the BCL-XL expression and mutation, and its cfinical significance in non-Hodgkin＇s lymphoma. Lymphoma cells were selectively isolated by laser microdissection. BCL-XL expression from lymphoma tissue and microdissected lymphoma cells was measured by using real-time quantitative reverse transcription- polymerase chain reaction. BCL-XL mutation was analyzed by using direct sequencing of PCR products. The results showed that compared to 15 patients with reactive hyperplasia, BCL-XL was overexpressed in follicular lymphoma （n = 30）, both in lymphoma tissue （P =0. 0064） and in microdissected lymphoma cells （P 〈0.0001 ）. No significant rise of BCL-XL expression was observed in patients with T-cell lymphoma （ n = 24） and diffuse large B cell lymphoma （ n = 24 ）. In follicular lymphoma, high BCL-XL level was associated with multiple extranodal involvement （ P = 0. 0004）, elevated lactate dehydrogenase level （P = 0. 0019）, high-risk international prognostic index （P = 0. 0013 ） and a short overall survival time （P =0.0451 ）. Mutation analysis revealed one synonymous mutation （ Codon 109 ACA→ACC） in one case of follicular lymphoma patient. It is concluded that BCL-XL expression is closely correlated with progress of follicular lymphoma and prognosis of patients with follicular lymphoma. The value of BCL-XL expression as a prognostic marker in follicular lymphoma should be considered.

关 键 词：非霍奇金淋巴瘤 BCL-XL基因 基因表达 基因突变 

分 类 号：R733.1[医药卫生—肿瘤]