热带假丝酵母及其重组菌产十三碳二元酸的补糖发酵实验  

Sucrose-added Fed-batch Culture for 1,11-Dicarboxylic Acid Production by Candida tropicalis and Recombinant Candida tropicalis Strain

作　　者：高弘[1] 刘铭[1] 黄英明[1] 华玉涛[1] 曹竹安[1] 

机构地区：[1]清华大学化学工程系生物化工研究所,北京100084

出　　处：《过程工程学报》2006年第5期814-817,共4页The Chinese Journal of Process Engineering

基　　金：国家自然科学基金资助项目(编号:29976022;30000003);国家重点基金资助项目(编号:20036010);国家重点基础研究发展规划(973)基金资助项目(编号:2003CB716007)

摘　　要：利用热带假丝酵母原始菌F10-1及已构建的肉毒碱乙酰转移酶基因单拷贝缺失重组菌CZ-15,在5L发酵罐中进行了补糖发酵批式培养,生产十三碳二元酸(DCA13).结果表明,肉毒碱乙酰转移酶基因单拷贝缺失后,CZ-15菌株的最佳补糖时间和补糖浓度都与原始菌F10-1不同.采用补糖工艺后,F10-1与CZ-15的十三碳二元酸的产量分别提高了25.0%和23.4%,达到99.5和108.1g/L,烷烃转化率分别提高了35.5%和28.6%.Candida tropicalis strain F 10-1 and its recombinant strain CZ-15 can be used for 1,11-dicarboxylic acid （DCA 13） production, and in CZ-15, one copy of carnitine acetyltransferase （CAT） genes is disrupted. The sucrose-added fed-batch fermentation of F 10-1 and CZ-15 was studied. The results showed that the optimal feeding time and the optimal sucrose concentration were different for F10-1 and CZ-15. By feeding sucrose in the acid production stage, for F10-1 and CZ-15, the acid production was increased by 25.0% and 23.4%, reaching 99.5 and 108.1 g/L, and the alkane conversion rate was increased by 35.5% and 28.6%, respectively.

关 键 词：肉毒碱乙酰转移酶 十三碳二元酸 热带假丝酵母 基因工程菌 补糖发酵 

分 类 号：TQ929[轻工技术与工程—发酵工程]