桑黄胞内多糖液体发酵培养基的优化  

Optimization for Medium Compositions for Intracellular Polysaccharide of Phellinus baumii in Submerged Culture

作　　者：李朔[1] 丁一新[1] 徐霁[1] 李玉祥[1] 赵明文[1] 

机构地区：[1]南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室,江苏南京210095

出　　处：《食品科学》2006年第11期236-240,共5页Food Science

基　　金：国家自然科学基金项目(30300006;30571294)

摘　　要：本文对桑黄的液体发酵培养进行了研究,用L27(313)表进行四因素三水平正交试验,筛选出了较好的桑黄胞内多糖高产液体发酵培养基,产量达到2.70g/L,其配方为初始pH5.0、黄豆粉、可溶性淀粉、C/N为1:1;通过直观分析和方差分析,胞内多糖含量的主控因素初始pH(A)与菌丝干重的主控因素氮源(B)之间是相互独立的,两者结果的相关分析得出r=-0.16(p>0.05),表明胞内多糖含量与菌丝干重之间没有可靠的相关性。The influence of liquid culture conditions on intracellular polysaccharide （IPS） production of PheUinus baumii was investigated. According to the orthogonal array design, the relative optimum medium compositions for IPS production were initial pH5.0, soybean powder, soluble starch, C/N ratio of 1：1, the production reached 2.70g/L. According to Intuitive analysis and variance analysis, the main factor of IPS was initial pH （A）, the main factor of mycelial dry weight was nitrogen （B）, A and B were independence with each other, correlation coefficient of the results of IPS content and mycelial dry weight was - 0.16 （p 〉 0.05）, it showed there wasn＇t credibility pertinence between IPS content and mycefial dry weight.

关 键 词：桑黄 正交设计 胞内多糖(IPS) 菌丝干重 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]