Construction of Human ScFv Phage Display Library against Ovarian Tumor  

作　　者：夏劲松 毕昊 姚琴 屈伸 宗义强 

机构地区：[1]Department of Nuclear Medicine,Tongji Hospital,Tongji Medical College,Huazhong Universty of Science and Technology,Wuhan 430030,China [2]Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2006年第5期497-499,共3页华中科技大学学报（医学英德文版）

基　　金：This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30200295).

摘　　要：In order to construct a single chain fragment variable （ScFv） phage display library against ovarian tumor, by using RT-PCR, the human heavy chain variable region genes （VH） and light chain variable region genes （VL） were amplified from lymphocytes of ovarian tumor patients and subsequently assembled into ScFv genes by SOE. The resulting ScFv genes were electrotransformed into E. coli TG1 and amplified with the co-infection of helper phage M13KO7 to obtain phage display library. The capacity and titer of the resulting library were detected. The phage antibody library with a capacity of approximately 3 × 10^9 cfu/μg was obtained. After amplification with helper phage, the titer of antibody library reached 5 μ 10^12 cfu/mL. Human ScFv library against ovarian tumor was constructed successfully, which laid a foundation for the screening of ovarian tumor specific ScFv for the radioimmunoimaging diagnosis of ovarian tumor.In order to construct a single chain fragment variable （ScFv） phage display library against ovarian tumor, by using RT-PCR, the human heavy chain variable region genes （VH） and light chain variable region genes （VL） were amplified from lymphocytes of ovarian tumor patients and subsequently assembled into ScFv genes by SOE. The resulting ScFv genes were electrotransformed into E. coli TG1 and amplified with the co-infection of helper phage M13KO7 to obtain phage display library. The capacity and titer of the resulting library were detected. The phage antibody library with a capacity of approximately 3 × 10^9 cfu/μg was obtained. After amplification with helper phage, the titer of antibody library reached 5 μ 10^12 cfu/mL. Human ScFv library against ovarian tumor was constructed successfully, which laid a foundation for the screening of ovarian tumor specific ScFv for the radioimmunoimaging diagnosis of ovarian tumor.

关 键 词：ScFv phage display library ovarian tumor SOE RADIOIMMUNOIMAGING 

分 类 号：R737.31[医药卫生—肿瘤]