Anticancer Activities of Trichostatin A on Maligant Lymphoid Cells  

作　　者：孙春艳 刘新月 陈燕 刘芳 

机构地区：[1]Institute of Hematology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2006年第5期538-541,共4页华中科技大学学报（医学英德文版）

基　　金：This project was supported by a grant from National Natural Sciences Foundation of China (No. 30271672).

摘　　要：The anticancer activity of trichostain A （TSA） on human B cell non-Hodgkin＇s lymphoma and its mechanism were explored, The effect of TSA on the growth of Raji cells and normal peripheral blood mononuclear cells （NPBMNC） was studied by MTr assay, The effect of TSA on the apoptosis of Raji cells and NPBMNC was studied by flow cytometry and TDT-mediated dUTP nick end labeling （TUNEL）. The effect of TSA on the cell cycle of Raji cells was studied by propidium iodide method. The results showed that TSA potently inhibited proliferation of Raji cells at microgram concentrations and induced apoptosis of Raji cells in a time-and concentration-dependent manner. Treatment with TSA induced accumulation of cells in G0/G1 or G2/M and a concomitant decrease of cell population in S phase. However, NPBMNC was less sensitive to the cytotoxic effect of TSA than Raji cells. It was concluded that TSA may inhibit the proliferation of Raji cells by regulating the cell cycle and inducing the cell apoptosis. Moreover, TSA demonstrates low toxicity in NPBMNC but selectively induces apoptosis of Raji cells.The anticancer activity of trichostain A （TSA） on human B cell non-Hodgkin＇s lymphoma and its mechanism were explored, The effect of TSA on the growth of Raji cells and normal peripheral blood mononuclear cells （NPBMNC） was studied by MTr assay, The effect of TSA on the apoptosis of Raji cells and NPBMNC was studied by flow cytometry and TDT-mediated dUTP nick end labeling （TUNEL）. The effect of TSA on the cell cycle of Raji cells was studied by propidium iodide method. The results showed that TSA potently inhibited proliferation of Raji cells at microgram concentrations and induced apoptosis of Raji cells in a time-and concentration-dependent manner. Treatment with TSA induced accumulation of cells in G0/G1 or G2/M and a concomitant decrease of cell population in S phase. However, NPBMNC was less sensitive to the cytotoxic effect of TSA than Raji cells. It was concluded that TSA may inhibit the proliferation of Raji cells by regulating the cell cycle and inducing the cell apoptosis. Moreover, TSA demonstrates low toxicity in NPBMNC but selectively induces apoptosis of Raji cells.

关 键 词：Trichostatin A Raji cell APOPTOSIS Cell cycle CYTOTOXICITY 

分 类 号：R733.1[医药卫生—肿瘤]