树突状细胞在S-180肉瘤模型鼠体内的分布和细胞间黏附分子-1等因子的表达  

THE DISTRIBUTION AND EXPRESSION OF INTERCELLULAR ADHESI ON MOLECULE-1 ET AL FACTORS OF DC IN THE MURINE S-180 SARCOMA MODEL IN VIVO

作　　者：谢遵江[1] 贺业春[1] 贾立敏[1] 郑金华[1] 刘颖[1] 刘丽[1] 张亿倬[1] 

机构地区：[1]哈尔滨医科大学解剖学教研室,哈尔滨150086

出　　处：《解剖学报》2006年第5期530-534,共5页Acta Anatomica Sinica

基　　金：黑龙江省自然科学基金资助项目(D0315)

摘　　要：目的通过研究小鼠树突状细胞在体内的迁移、分布、形态特征及一些因子的表达情况,探讨黏附分子和趋化因子在S-180肉瘤模型鼠体内的表达及其在树突状细胞迁移过程中的调控作用。方法采用光镜、透射电镜、免疫组织化学和原位杂交染色方法,观察肿瘤模型鼠中树突状细胞的分布和形态学特征;分析上皮型钙黏附分子(E-cadherin)、细胞间黏附分子-1(ICAM-1)、单核细胞趋化蛋白-1(MCP-1)、T细胞特异性趋化因子(Rantes)、巨噬细胞炎性蛋白-1β(MIP-1β)和趋化因子受体7(CCR7)的表达情况,并与对照组进行比较。结果树突状细胞在肿瘤模型鼠中,分布于肿瘤局部和腋窝淋巴结(ALN),在数量上与对照组存在明显差异,其ICAM-1、MCP-1、Rantes、MIP-1β和CCR7表达明显上调,E-cadherin的表达下降;分别与对照组数密度和面密度比较有显著差异。结论在肿瘤抗原诱导下,ICAM-1、MCP-1、Rantes、MIP-1β和CCR7表达升高,有趋化树突状细胞向淋巴组织或肿瘤局部迁移和聚集的作用;而E-cadherin表达下降可能有利于树突状细胞的迁移。Objective To study the migration, distribution and morphological characteristics of DC in vivo, to study the expressions of adhesion molecules and chemokines in the murine S-180 sarcoma model, and their regulative role for DC in the migration. Methods By the method of innnunohistochemistry and in situ hybridization stainings, light microscope and electron microscope, We observed the distributive and morphological characteristics of DCs in vivo in the murine S-180 sarcoma model. The expressions of E-cadherin, ICAM-1, MCP-1, Rantes, MIP-1β mRNA and CCR7 mRNA were compared with control group. Results The DCs in vivo mainly distributed in the tumor-surrounding tissue and ALN of murine tumor model. The number of DCs was larger in the experiment group than that in control group. The expressions of ICAM-1, MCP-1, Rantes, MIP-1β mRNA and CCR7 mRNA were higher, but lower of E-cadherin in the murine tumor model. The number and area density of experiment group was greater compared with that of control group. Conclusion These results suggested that the high expressions of ICAM-1, MCP- 1, Rantes, MIP-1β mRNA and CCR7 mRNA may induce DCs to migrate and gather into lymphatic tissues or tumor-surrounding tissues in the murine tumor model, but the low expressions of E-cadherin may make for the migration of DCs.

关 键 词：树突状细胞 黏附分子 趋化因子 免疫组织化学 原位杂交 肿瘤鼠模型 

分 类 号：R338.21[医药卫生—人体生理学]