甘露聚糖修饰的重组小鼠甲胎蛋白的抗肿瘤免疫作用研究  

Investigation of Role in Antitumor Immune of Mouse Recombinant Alpha-fetoprotein Conjugated with Mannan

作　　者：张巍[1] 魏于全[1] 陈俐娟[1] 刘继彦[1] 吴扬[1] 丁振宇[1] 文艳君[1] 杨金亮[1] 范玲玉[1] 李炯[1] 阚兵[1] 邓洪新[1] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室,成都610041

出　　处：《四川大学学报（医学版）》2006年第6期830-834,共5页Journal of Sichuan University(Medical Sciences)

摘　　要：目的研究用甘露聚糖修饰后的甲胎蛋白(AFP)对肿瘤免疫的促进作用。方法用小鼠建立原发性肝癌模型,以AFP作为模型抗原,将重组小鼠AFP(mAFP)与氧化型的甘露聚糖相连接制成疫苗(Ox-M-mAFP),同时用未与甘露聚糖连接的mAFP作为对照,研究该疫苗在小鼠体内的肿瘤免疫治疗效果。结果Ox-M-mAFP可在小鼠体内诱导出有效的抗肿瘤免疫反应,免疫后小鼠脾内得到的CD8+T淋巴细胞显示出对特异性分泌AFP的肿瘤细胞的杀伤活性。结论本实验提供了一种有效激活机体针对自身分子免疫应答的肿瘤疫苗策略,也对原发性肝癌的非手术治疗提供了一个可能的研究方向。Objective To investigate how to deliver AFP to the immune system for generating the appropriate immune response. Methods We prepared the oxidized mannan linked to recombinant mouse α-fetoprotein （mAFP） as a vaccine （Ox-M-mAFP）, and the vaccine based on mAFP without mannan modification was also prepared as a control. Results We found that immunotherapy with Ox-M-mAFP was effective on protective and therapeutic antitumor immunity in mice. No marked toxicity was found in the immunized mice. Conclusion These findings may be important to further exploration of the breaking of immune tolerance to self molecules, and may provide a new vaccine strategy for the treatment of AFP-positive hepatocellular carcinoma.

关 键 词：疫苗 甲胎蛋白 原发性肝癌 甘露聚糖 

分 类 号：R73-36[医药卫生—肿瘤]