强迫谱系障碍与第Ⅱ组代谢型谷氨酸受体相关候选基因位点的连锁分析  

A linkage analysis between obsessive compulsive spectrum disorders and candidate genes of group Ⅱ metabotropic glutamate receptor

作　　者：徐西嘉[1] 陈晓岗[1] 唐劲松[1] 郭文斌[1] 赵靖平[1] 胡正茂[2] 

机构地区：[1]中南大学湘雅二院精神卫生研究所 [2]中南大学医学遗传学国家重点实验室

出　　处：《中国行为医学科学》2006年第11期967-969,共3页Chinese Journal of Behavioral Medical Science

基　　金：国家自然科学基金重点项目(60433020)

摘　　要：目的了解代谢型谷氨酸受体mGlu2受体(mGluR2)基因、mGlu3受体(mGluR3)基因等与强迫谱系障碍(OCSDs)的连锁关系。方法选取一个连续三代发病的强迫谱系障碍家系,采集到该家系中12个正常个体,8个受累个体的血样,选取mGluR2、mGluR3基因附近7对微卫星标记引物,采用两点和多点非参数分析的方法对该家系进行连锁分析。结果7对微卫星标记位点的两点和多点非参数分析LOD值(NPL值),位于mGluR3基因附近的标记D7S644两点非参数分析NPL值为1.719(P=0.078),多点非参数分析NPL值为1.712(P=0.078),达到验证性连锁的阈值(NPL=1.2),但未达到提示性连锁的阈值(NPL=2.2),D7S2481两点非参数分析NPL值为0.628(P=0.179),多点非参数分析NPL值为0.852(P=0.141),接近验证性连锁的阈值,其余5对微卫星标记位点非参数分析NPL值均未达到验证性连锁的阈值。结论提示mGluR3基因与OCSDs可能存在连锁关系,不能排除mGluR2基因与OCSDs的相关性。Objective To investigate the linkage relationship between mGluR2 ,mGluR3 gene locus and obsessive compulsive spectrum disorders. Methods Altogether 20 subjects from a family were recruited, including 8 patients and their relatives. The subjects were interviewed with Dignostic Interview for Genetic Studies （DIGS） and Family Interview for Genetic Studies（FIGS）. All enrolled patients fulfilled the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition （DSM-Ⅳ） diagnosis of obsessive compulsive spectrum disorders. Seven microsatellite polymorphisms from mGluR2, mGluR3 gene region were determined after the polymerase chain reaction. Nonparametric linkage analysis was performed with GENEHUNTER software version 2.1. Results The two-point NPL score of D7S644 was 1.719 （ P = 0. 078 ）, the multipoint NPL score of D7S644 was 1.712 （ P = 0. 078 ）. The two-point NPL score of D7S248 t was 0. 628 （ P = 0. 179） ,the muhipoint NPL score of D7S2481 was 0. 852 （ P = 0. 141 ）. The NPL score of the other five microsatellite markers did not reach 1.2, which was the liminal value of indicative linkage. Conclusion The data suggest mGluR3 gene may be associated with obsessive compulsive spectrum disorders.

关 键 词：强迫谱系障碍 代谢型谷氨酸受体 连锁遗传学 

分 类 号：R749.7[医药卫生—神经病学与精神病学]