一株菲降解细菌的分离鉴定及其特性  

Isolation and identification of a phenanthrene-degrading bacterial strain.

作　　者：祝儒刚[1] 钟鸣[1] 周启星[2] 刘海宁[1] 李玉双[1] 

机构地区：[1]沈阳农业大学辽宁省农业生物技术重点实验室,沈阳110161 [2]中国科学院沈阳应用生态研究所陆地生态过程重点实验室,沈阳110016

出　　处：《应用生态学报》2006年第11期2117-2120,共4页Chinese Journal of Applied Ecology

基　　金：国家"863"计划项目(2004AA649060);中国科学院陆地生态过程重点实验室开放课题资助项目.

摘　　要：通过选择性富集培养,从沈抚灌区石油污染土壤中分离到1株菲降解细菌.试验证明该菌株能以菲为唯一碳源和能源生长.经形态学、生理生化鉴定和16SrRNA基因序列比对分析,确定该菌株属于不动杆菌属,命名为Acinetobactersp.L2.系统发育进化分析发现,L2菌株与Acinetobactersp.DG880[AY258108]亲源关系最近.L2菌株培养7d后对菲的降解率达96·3%.邻苯二酚2,3-双加氧酶活力测定表明,L2菌株可能含有菲降解基因.Through selective enriched culture, a phenanthrene-degrading bacterial strain was isolated from the oil-contaminated soil in Shenfu irrigation area of Shenyang, Northeast China. The morphological and physiological-biochemical identification, 16S rDNA sequence analysis, and phylogenetic study showed that this strain was belonged to genus Acinetobacter and named as Acinetobacter sp, L2, and closest to Acinetobacter sp. DG880[ AY258108 ]. It could use phenanthrene as the sole carbon source, Mter 7 days culture, the degradation rate of phenanthrene was 96. 3%. According to the activity of catechol 2,3-dioxygenase, the strain probably had phenanthrene-degrading genes.

关 键 词：菲 16SrRNA ACINETOBACTER 生物降解邻苯二酚2 3-双加氧酶 

分 类 号：X172[环境科学与工程—环境科学]