Cyclin D1在食管癌中的表达及其临床意义  

Cyclin D1 Expression in Esophageal Cancer and Its Clinical Significance

作　　者：张旭[1] 黄晓平[1] 马国伟[1] 苏晓东[1] 朱志华[1] 戎铁华[1] 

机构地区：[1]华南肿瘤学国家重点实验室//中山大学肿瘤防治中心胸科,广东广州510060

出　　处：《中山大学学报（医学科学版）》2006年第6期657-660,693,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金资助项目(30572103);广东省自然科学基金资助项目(5300808);中国博士后科学基金资助项目(2005037612)

摘　　要：【目的】检测食管癌中CyclinD1的表达并探索其临床意义。【方法】运用RT-PCR与组织芯片免疫组化法检测CyclinD1在食管癌组织、配对的邻近食管上皮中的表达状态。分析探讨CyclinD1的表达状态与食管癌临床和病理参数之间的关系。【结果】61%和35%的病例分别在食管鳞癌及其邻近上皮组织中显示CyclinD1表达增加。食管癌组织与其邻近的上皮组织之间CyclinD1的表达具有显著差异(!2=8.776,P<0.01)。不同T分期的肿瘤组织中CyclinD1的表达差异具有统计学意义,在T3+T4期的表达率为70%,T1+T2期的表达率为40%(T3+T4vsT1+T2,!2=5.363,P<0.05);在T3期的表达率为70%,T1期的表达率为30%(T3vsT1,!2=4.646,P<0.05)。【结论】CyclinD1可能是参与食管癌化的分子标志物,并且可能是区分不同食管癌T分期的有价值的分子标志物。[Objective] Cyclin D1 expression in esophageal carcinomas were detected and its clinical significance were explored. [Method] The expression status of cyclin D1 in esophageal squamous cell carcinomas tissue and matched adjacent epithelia were investigated by RT-PCR and tissue microarray immunohistoehemistry analysis. The relationship between the expression status of cyclin D1 and the clinical and pathological parameters of esophageal cancer were explored. [Result] 61% and 35% cases showed increased expression of cyclin D1 in esophageal carcinomas and the adjacent epithelia, respectively. Significant difference for cyclin D1 expression was found between esophageal carcinomas and the adjacent epithelia （x^2=8.776,P〈 0.01）. Comparing cyclin D1 expression in the carcinomas at different T stages, we found significant alterations. The expression rates were 70% at T3＋T4 stage and 40% at T1＋T2 stage（T3＋T4 vs TI＋T2, x^2 = 5.363, P〈 0.05）;the expression rate were 70% at T3 stage and 30% at T1 stage （T3 vs T1, x^2= 4.646, P〈 0.05）. [Conclusion] The results suggested that cyclin D1 might be a molecular marker in esophageal carcinogenesis and in distinguishing different T stage in esophageal cancer.

关 键 词：食管肿瘤 CYCLIN D1 组织芯片 分子标志物 

分 类 号：R735.1[医药卫生—肿瘤]