微孔板杂交检测乙肝病毒X基因及其对肝细胞癌诊断价值的研究  

Microwell plate pybridization technique to detect HBV X gene and its clinical application value of HCC

作　　者：宋玉国[1] 张吉林[2] 黄建文[1] 熊英[1] 毕胜利[2] 

机构地区：[1]北华大学附属医院,吉林吉林132011 [2]北华大学医学院

出　　处：《中国实验诊断学》2006年第11期1348-1349,共2页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技厅资助项目(19990316-03)

摘　　要：目的建立微孔板杂交技术检测乙肝病毒X基因的方法,研究其在肝细胞癌诊断中的应用价值。方法在微孔板上包被HBV X基因片段的捕获探针;应用Bio-11-dUTP修饰的HBV X基因扩增引物,对待测标本进行PCR扩增,在微孔板上杂交后用链霉亲和素碱性磷酸酶系统显色。结果成功建立了微孔板杂交技术检测HBV X基因的方法;乙肝病毒感染后肝细胞癌患者X基因阳性检出率明显高于慢性乙肝患者和肝纤维化患者(P<0.01)。结论检测HBV X基因对肝细胞癌具有辅助诊断价值。Objectlve To set up hybridization technique on micrawen plate to detect HBV X gene, invest to clinical application value on HCC. Methods On microwell plate capture probe was coated HBV X gene frangment; Gene which was modified by Bio-11- dUTP amplified primer. PCR amplified objective fragment of examined sample. After hybridization on microweU plate, using streptavidin ALP system colorated. Results It is succcessful to set up hybridization techmiquc on microwell plate to detect HBV X. The method detected HBV X sensitiveness and idio;X gene of positive rate of HCC patients is obviously more than that of chronic serm hepatitis and hepatic fibrosis（ P 〈 0.01）. Conclusion Detecting HBV X gene possess auxiliary diagnosis value for HCC.

关 键 词：乙肝病毒 X基因 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]