紫外分光光度法测定甜蜜素  

Determination of sodium cyclamate by ultraviolet spectrophotometry

作　　者：桑宏庆[1] 于秋生[1] 

机构地区：[1]安徽科技学院,安徽凤阳233100

出　　处：《饮料工业》2006年第11期28-30,共3页Beverage Industry

基　　金：安徽省教育厅自然科学研究项目(2006KJ032C);安徽科技学院自然科学课题(2006YZ011)

摘　　要：在硫酸介质中用乙醚提取食品中的甜蜜素,样品溶液的吸光度在一定范围内与甜蜜素的含量有线性关系,建立了测定甜蜜素的新方法。该法最大吸收波长为209nm,表观摩尔吸光系数为2.29×103L·mol-·1cm-1,甜蜜素浓度在0～0.7g/L范围内符合比耳定律,最小检出限为0.04g/L,相对标准偏差为2.84%～3.46%,回收率为90.0%～102.8%。It is found that in sulfuric acid medium, when extracting sodium cyclamate in food by diethyl ether, the absorbance of samples is linear to the amount of sodium cyclamate present in a certain rartge. Based on these facts, a new ultraviolet spectrophotometric method for the detemaination of sodium cyclamate is proposed in this paper .The absorpttive maximum lies at 2Ognm with an apparent molar absorptivity of 2.29×10^3 L·mol^-1·cm^-1 Beer＇s law is obeyed in the concentration range from 0 to 0.7g/L, the detection limit of this method is 0.04g/L and the relative standard deviation is 2.84%-3.46%. Recoveries obtained by the standard addition method were in the range of 90.0%-102.8%.

关 键 词：紫外分光光度法 甜蜜素 测定 

分 类 号：TS207.3[轻工技术与工程—食品科学]