酒酒球菌的快速特异性PCR鉴定  

Rapid Specificity Identification of Oenococcus oeni with PCR

作　　者：刘延琳[1] 李华[1] 

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100

出　　处：《微生物学杂志》2006年第6期26-29,共4页Journal of Microbiology

基　　金：陕西省自然基金(2003C105);西北农林科技大学青年学术骨干支持计划资助(01140301)

摘　　要：以Oenococcus oeni苹果酸-乳酸酶基因(mleA)为目标基因,设计了1对特异性引物PmleaL/PmleaR进行酒酒球菌的快速鉴定研究。结果表明,直接以O.oeni的菌落为模板,通过引物对PmleaL/PmleaR的PCR扩增,可得到mleA基因的特异性条带;用此特异性引物进行供试乳酸菌的PCR鉴定,所有O.oeni菌系均得到特异性条带,而供试的其它种类乳酸菌未扩增出目标带。PmleaL/PmleaR可用于O.oeni的快速PCR鉴定。A malolactic enzyme （ role A） as target gene for designing a rapid identification of Oenococcus oeni with a pair of specific primers of PmleaL/PmleaR was studied. The results indicated that specific band of role A gene was obtained through the PCR amplification of primer pair of PmleaL/PmlcaR from colonies of O. oeni as template directly. And with this specific primer to carry out the identification of lactic acid bacteria for test, specific band of role A gene was obtained from all O. oeni but failed to obtain from other genera of lactic acid bacteria for test. The primers of PmleaL/PmleaR are specific to O. oeni and can be used for rapid identification of O. oeni.

关 键 词：酒酒球菌 苹果酸-乳酸酶基因 特异引物 快速鉴定 

分 类 号：TS262.6[轻工技术与工程—发酵工程]