阪崎肠杆菌的生物学特性及其检测技术  

The Biologic Characteristics of Enterobacter sakazakii and its Detection Techniques

作　　者：吴清平[1] 叶应旺[1,2,3] 郭伟鹏[1] 杨宁[4] 

机构地区：[1]广东省微生物研究所广东省菌种保藏与应用重点实验室 [2]中国科学院南海海洋研究所广州510301 [3]中国科学院研究生院北京100049 [4]广东环凯微生物科技有限公司

出　　处：《微生物学通报》2006年第6期99-103,共5页Microbiology China

基　　金：广东省自然科学基金资助项目(No.04000244)

摘　　要：阪崎肠杆菌(Enterobactersakazakii)是寄生于人和动物肠道的条件性肠道致病菌,能引起新生儿脑膜炎,致死性小肠结肠炎以及菌血症等,具有产生α-葡萄糖苷酶和吐温80脂酶、不发酵三梨醇以及无磷酸胺酶活性等生理生化特征,并且对干燥、渗透压和抗生素等有很强的抗性。该菌自然来源非常广泛,在水、土壤、植物根茎、动物肠道甚至加工食品都可存在,其中婴儿配方奶粉是婴儿感染阪崎肠杆菌的主要渠道。近年来基于α-葡萄糖苷酶反应的特异性生化检测和PCR扩增的分子检测技术已取得重要进展,但是目前还缺乏一种稳定检测该菌的分子分型检测技术。Enterobacter sakazakii is an opportunistic pathogen parasized in human and animal intestines causing meningitis, sepsis and necrotizing enterocolitis and proudcing α-glucosidase and tween-80 esterase, but not fermenting D-sorbitol and no phosphoamidase acvitity. It also shows resistance to antibiotic, osmosis and desiccation. The food-borne pathogen could exist in the soil, water, rootstock of plants, intestine of animals and machining foods. Furthermore, the powered infant formula milk was the main infection sources. The special biochemical detection based on ,α- glucosidase reaction and molecular detection techniques based on PCR amplification have been made much progress in recent years, but in much need of a stable molecular typing technique for de tection of Enterobacter sakazakii.

关 键 词：阪崎肠杆菌 生物学特性 检测技术 Α-葡萄糖苷酶 

分 类 号：R115[医药卫生—公共卫生与预防医学]