Celecoxib对胃癌细胞凋亡的影响  

Effect of Celecoxib on apoptosis of human gastric cancer cell line

作　　者：凌桂琴[1] 王绪[2] 王琳[3] 李小翠[4] 

机构地区：[1]徐州医学院第二附属医院肿瘤内科,江苏省徐州市221006 [2]徐州医学院医学影像学系,江苏省徐州市221002 [3]徐州医学院组胚教研室,江苏省徐州市221002 [4]徐州医学院微生物学教研室,江苏省徐州市221002

出　　处：《世界华人消化杂志》2006年第34期3302-3305,共4页World Chinese Journal of Digestology

摘　　要：目的:研究选择性环氧合酶-2(COX-2)抑制剂Celecoxib对人胃癌细胞株SGC7901凋亡的影响.方法:采用MTT法测定人胃癌细胞株SGC7901分别在1×10-5,1×10-6,1×10-7,1×10-8,1×10-9mol/L的Celecoxib作用48h后生长抑制情况;流式细胞术(FCM)观察Celecoxib对SGC7901细胞凋亡的影响;采用免疫细胞化学染色观察Survivin的表达.结果:Celecoxib浓度为1×10-5-1×10-9mol/L时,对SGC7901细胞的生长均有抑制作用,以1×10-5mol/L浓度的抑制作用最为显著,其抑制率为30.03%;1×10-5mol/LCelecoxib作用12,24,48h后,细胞凋亡比例较对照组均明显增加,48h凋亡率达17.83%;1×10-5mol/LCelecoxib作用后,Survivin的表达自用药3h起下降,24h最明显.结论:Celecoxib可诱导SGC7901细胞的凋亡,其可能的作用机制为抑制细胞Survivin基因的表达.AIM： To explore the effect of Celecoxib （cyclooxygenase-2 inhibitor） on the apoptosis of gastric cancer cell line SGC7901 in vitro. METHODS： After gastric cancer cell line SGC7901 was treated with Celecoxib （1×10^-5 to 1×10^-9 mol/L） for 24 hours, the proliferration of cells was detected by MTT assay. Flow cytometry （FCM） was used to measure the apoptosis of SGC7901 cells, and immunocytochemistry was used to observe the expression of Survivin. RESULTS： Celecoxib inhibited the proliferation of SGC7901 cells at the concentrations of 1×10^-5 to 1×10^-9 mol/L, and the maximal inhibitory rate was 30.03%, which was produced by 1×10^-5 mol/ L Celecoxib. After SGC7901 cells were treated with 1×10^-5 mol/L Celecoxib for 12, 24, 48 hours, the proportions of apoptosis cells were signifi-cantly increased in comparison with those of the control cells, and the apoptosis rate was 17.83% after 48 hours. The expression of Survivin was decreased at 3 and 24 hours, especially at 24 hours. CONCLUSION： Celecoxib can induce the apoptosis of gastric cancer cell line SGC7901 by suppressing the expression of Survivin.

关 键 词：胃癌 CELECOXIB 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]