X射线对肺癌细胞系p57^(kip2)转录表达的影响  

Effect of x-irradiation on the transcription of p57^(kip2) in lung cancer cell lines

作　　者：邹华伟[1] 谭永刚[1] 朱喜科[2] 李凯[1] 韩琤波[1] 张贺英[1] 徐茹[1] 

机构地区：[1]中国医科大学盛京医院肿瘤科,辽宁沈阳110004 [2]中国医科大学盛京医院遗传室,辽宁沈阳110004

出　　处：《中华肿瘤防治杂志》2006年第20期1538-1540,1544,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:研究X射线对肺癌细胞系NCI-H226和A549的印记基因p57kip2转录表达的影响。方法:对数生长期的肺癌细胞系NCI-H226和A549,分别予以2、4、8和12GyX射线照射,并于照射前和照射后6、12、24、36和48h分别提取RNA,采用RT-PCR技术检测各时相p57kip2mRNA的含量,分析不同剂量X射线照射后对其转录水平的影响。结果:两组肺癌细胞p57的转录表达在不同照射剂量和不同时间水平间差异有统计学意义,P<0·05,A549经过X射线照射后,p57kip2的mRNA含量明显升高,且与照射剂量呈线性依赖关系;NCI-H226虽然未见明显规律,但在X射线照射后的峰值mR-NA含量均明显升高。结论:X射线照射后能够上调肺癌细胞p57kip2的mRNA的表达,预示着p57可能参与了放射线所致细胞周期重新分布和细胞凋亡的过程,并且可能预测肺癌细胞的放射敏感性,但其表达与时间剂量的关系尚需要进一步研究。OBJECTIVE： To study the effect of x-irradiation on the transcription of p57^kip2 in lung cancer cell lines. METHODS. Logarithmic growing phase of lung cancer cell lines NCI-H226 and A549 were irradiated under 2, 4, 8, 12 Gy respectively by 6 MV X-ray and RNA were extracted before x-irradiation and at 6 h, 12 h, 24 h, 36 h, 48 h after x-irradiation. The level of p57 mRNA was detected with RT-PCR to show the effect of x-irradiation on the transcription of p57. RESULTS： The level of p57 mRNA was very low before X-irradiation, and increased significantly after irradiation by different doses. In this study we found that the level of p57 mRNA in cell line A549 increased with the increased radiation dose and reached to the peak level at 24 hours after irradiation when irradiated by 2-8 Gy. Though p57 mRNA level of A549 cell line subjected by an irradiation dose of 12 Gy increased, yet it was lower than that subjected by 2-8 Gy dose, and the peak time was prolonged to 36 hours. There was no obvious tendency found in the NCI-H226 cell line, but the level of t357 mRNA was increased in different radiation dose group with statistical significance. CONCLUSION.. X-irradiation could up-regulate the level of p57 mRNA with a dose-dependant relationship, which might suggest that p57 take part in the course of cell cycle distribution and cell apoptosis induced by X-irradiation, and may be a predictor of radiosensitivity.

关 键 词：X线 细胞系 P57^KIP2 基因表达 肺肿瘤/放射疗法 

分 类 号：R734.2[医药卫生—肿瘤]